在新生儿重症监护病房中实施基于PCR的策略,以控制Serratia marcescens的爆发
Ángel Rodríguez-Villodres1,2,3, José Manuel Ortiz de la Rosa4,5, Raquel Valencia-Martin6,7
1Clinical Unit of Infectious Diseases, Microbiology and Parasitology, University Hospital Virgen del Rocío, Av. Manuel Siurot s/n, 41013, Seville, Spain. anrovi1797@gmail.com.
Annals of clinical microbiology and antimicrobials
|December 12, 2023
概括
一个新的实时PCR (qPCR) 策略有效控制了新生儿重症监护室 (NICU) 中的Serratia marcescens疫情. 这种分子监测减少了血液感染 (BSI),并防止了进一步的医院传播.
科学领域:
- 传染病流行病学 传染病流行病学
- 分子诊断学 分子诊断
- 医疗保健相关的感染
背景情况:
- 新生儿重症监护室 (NICU) 容易受到与医疗相关的感染.
- 塞拉蒂亚马尔塞森斯疫情对脆弱的新生儿构成重大风险.
- 有效的监测对于控制病房病原体传播至关重要.
研究的目的:
- 评估基于qPCR的新型监测策略的临床和流行病学影响.
- 评估qPCR在控制新生儿重症监护室内Serratia marcescens爆发的有效性.
- 确定疫情期间对血液感染 (BSI) 的影响.
主要方法:
- 开发一种特定的qPCR试验,用于检测直肠拭子中的Serratia marcescens.
- 两个监测期的比较:qPCR前 (qPCR疫情声明到qPCR引入) 和PCR期 (qPCR引入到疫情解决).
- 使用脉冲场凝电泳 (PFGE) 和追踪血液感染 (BSI) 的克隆分析.
主要成果:
- 在实施后,qPCR策略检测到16例新病例,而在qPCR前期间,该策略发现了19例新病例.
- PFGE在7个克隆群体中确定了24种不同的脉型,表明了多样化的菌株.
- 在qPCR实施后,BSI发作显著减少,从11个减少到1个,并且在18个月内没有进一步的爆发.
结论:
- 基于qPCR的分子监测策略在控制病房内Serratia marcescens在NICU中传播方面非常有效.
- 这种分子方法显著减少了临床影响,血流感染的急剧下降证明了这一点.
- 该研究强调了qPCR作为管理和预防重症监护机构格兰氏阴性细菌爆发的宝贵工具的实用性.
相关概念视频
Real Time RT-PCR
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Essential infection prevention measures are based on the knowledge of the infection chain, the modes of transmission in healthcare settings, and the use of the best practices in all healthcare settings. Compulsory public reporting of healthcare-associated infection rates is needed to allow individuals and the community to make informed choices regarding selecting a healthcare facility.
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