溶剂组成可以对小分子的离子移动性衍生的碰撞截面具有可测量的影响
Nadjali A Chung1,2,3, Jody C May1,2,3,4,5, Renã A S Robinson2,3
1Center for Innovative Technology, Vanderbilt University, Nashville, Tennessee 37235, United States.
Journal of the American Society for Mass Spectrometry
|December 12, 2023
概括
在离子流动性 (IM) 中的碰撞截面 (CCS) 测量取决于溶剂成分. 这项研究发现了微小但可测量的CCS依赖性,强调了在比较IM研究中报告溶剂系统的必要性.
科学领域:
- 分析化学 分析化学
- 物理化学 物理化学
背景情况:
- 离子流动性 (IM) 与液体色谱-质谱相结合,可在非向性研究中增强代谢物识别.
- 对碰撞横截面 (CCS) 测量的标准化对于在实验室和技术之间比较IM数据至关重要.
- 影响CCS的移动分离之外的因素仍未得到充分研究.
研究的目的:
- 为了研究水性:有机溶剂成分对碰撞截面 (CCS) 测量的影响.
- 为了评估CCS对各种化学标准的溶剂依赖性.
主要方法:
- 使用高精度漂流管仪器进行离子移动性测量.
- 在27种不同的水性:有机溶剂成分中分析了15种化学标准.
- 测量碰撞横截面 (CCS) 值是从第一原则中得出的.
主要成果:
- 观察到CCS对溶剂成分的微小可测量的依赖.
- 较大的分析样本 (m/z > 400) 在中间溶剂组合中 (40-60%) 显示CCS增加.
- 趋势与溶剂粘度和原质体行为有相似之处,尽管起源不清楚.
结论:
- 碰撞截面 (CCS) 受使用的水性有机溶剂系统的影响.
- 观察到的溶剂依赖,虽然很小 (<0.5%),但对于比较的离子流动性研究来说很重要.
- 报告溶剂成分对于离子流动性分析中准确和可重复的CCS数据至关重要.
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