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相关实验视频

Updated: Jul 8, 2025

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Published on: December 16, 2015

在C. elegans中进行细胞追踪,使用基于细胞位置热图的对齐和对对检测.

Kaito Shiku, Hiromitsu Shirai, Takeshi Ishihara

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference

|December 12, 2023

概括

这项研究引入了一种新的活体3D细胞追踪方法,专门针对C. elegans成像方面的挑战. 新方法提高了复杂生物样本中的细胞检测一致性和跟踪精度.

科学领域:

发育生物学发展生物学
生物医学成像技术生物医学成像技术
计算生物学计算生物学

背景情况:

精确的3D细胞跟踪对于发育生物学中活细胞图像分析至关重要.
在C. elegans中追踪细胞的挑战包括之间的大细胞迁移和由于图像质量和细胞接近而导致不一致的细胞检测.

研究的目的:

开发一种改进的C. elegans3D细胞追踪方法,克服现有技术的局限性.
提高活体C. elegans胚胎中细胞检测和跟踪的准确性和稳定性.

主要方法:

一种新的细胞跟踪方法,采用基于热图的非刚性对齐和测试时间微调的细胞位置.
一种双向检测方法,利用以前的检测结果来改善当前的细胞识别.

主要成果:

在拟议的细胞追踪方法中证明了单个模块的有效性.
与C. elegans3D细胞追踪现有方法相比,综合方法获得了更高的性能.

结论:

拟议的细胞追踪方法在挑战活体C. elegans成像场景时显著提高了准确性和稳定性.
非刚性对齐和双向检测的组合为复杂的3D细胞跟踪应用提供了强大的解决方案.

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C. elegans Tracking and Behavioral Measurement

C. elegans Tracking and Behavioral Measurement

Published on: November 17, 2012

Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans

Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans

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Last Updated: Jul 8, 2025

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

Published on: December 16, 2015

C. elegans Tracking and Behavioral Measurement

C. elegans Tracking and Behavioral Measurement

Published on: November 17, 2012

Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans

Worm-align and Worm_CP, Two Open-Source Pipelines for Straightening and Quantification of Fluorescence Image Data Obtained from Caenorhabditis elegans

Published on: May 28, 2020