纳米库尔库降低高葡萄糖和颗粒物引起的内皮炎症:线粒体功能和miR-221/222的参与
Tsai-Chun Lai1,2,3, Chiang-Wen Lee4,5,6, Mei-Hsiang Hsu1
1Department of Anatomy and Cell Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China.
International journal of nanomedicine
|December 12, 2023
概括
颗粒物和糖尿病通过增加线粒体损伤和炎症,使心血管疾病恶化. 纳米提供了一种潜在的治疗方法,通过逆转这些有害影响.
科学领域:
- 心血管研究研究心血管研究
- 环境健康 环境健康
- 分子生物学分子生物学
背景情况:
- 颗粒物 (PM) 2.5和糖尿病是心血管疾病 (CVD) 发病率和死亡率的重要危险因素.
- 关联联合PM和糖尿病暴露于心血管疾病的精确分子途径仍然不完全理解.
研究的目的:
- 研究由高葡萄糖 (HG) 和PM暴露引起的内皮炎症的分子机制.
- 评估纳米库尔库明 (NCur) 对高血压和PM诱导的内皮功能障碍的保护作用.
主要方法:
- 内皮细胞 (ECs) 暴露于HG和PM,有或没有NCur预处理.
- 评估了线粒体功能,活性氧物种 (ROS),炎症标志物 (ICAM-1) 和单细胞粘附.
- 采用了西方涂抹,免疫光和特定的分子分析 (例如,JC-1,MitoSOX).
- 在STZ诱导的高血糖小鼠身上也进行了实验.
主要成果:
- 同时暴露于HG和PM增加了ECs中的ICAM-1表达,单细胞粘附,线粒体ROS,线粒体裂变和线粒体衰变.
- NCur预治疗减轻了这些有害影响,并改善了内皮炎症.
- HG和PM诱导的炎症涉及到JNK通路和miR-221/222的向ICAM-1和BNIP3.
- 在高血糖小鼠中观察到类似的NCur保护作用.
结论:
- HG和PM暴露会通过线粒体功能障碍,氧化应激和线粒细胞衰变诱导内皮炎症.
- 纳米库尔库显示出对糖尿病和空气污染引起的内皮损伤的保护作用.
- 针对ICAM-1和BNIP3的miR-221/222通路与NCur.的治疗作用有关.
- 纳米为糖尿病和空气污染引起的心血管并发症提供了一个有前途的治疗策略.
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