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Collecting Variable-concentration Isothermal Titration Calorimetry Datasets in Order to Determine Binding Mechanisms
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通过连续注射异热定位热量计直接确定高亲和度结合常量

Ji Woong Chang1,2, Yanyu Mu1, Antonios Armaou1,3,4

  • 1Department of Chemical Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, United States.

The journal of physical chemistry. B
|December 12, 2023
PubMed
概括

连续注射方法异热定位热量计 (CIA-ITC) 准确地测量了受体结合的亚纳米分离常数 (KD). 这种无标签方法确定了desthiobiotin-avidin KD为6.5 pM,这对于其他技术来说是一项具有挑战性的壮举.

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