静脉内皮细胞生成和循环的动态在恒温和疾病期间
Yi Li1,2, Zixin Liu2, Ximeng Han1,2
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiaotong University School of Medicine, China (Y.L., X. Han, F.L., X.S., H.H., L.S., B.Z., B.H.).
Circulation
|December 12, 2023
概括
研究人员开发了一种基因系统来追踪动脉内皮细胞 (EC) 的增殖. 这项研究揭示了具有明显增殖能力的EC亚群,为血管修复提供了潜在的治疗点.
科学领域:
- 血管生物学
- 细胞动力学
- 复原医学
背景情况:
- 内皮细胞的自我增殖对于血管的修复和再生至关重要.
- 了解EC生成动态对于血管平衡和疾病研究至关重要.
- 在大动脉等大血管中评估EC增殖是具有挑战性的,因为细胞分裂很少发生.
研究的目的:
- 开发一个精确的基因系统,在体内记录EC的增殖动态.
- 研究动脉中EC生成的细胞和分子机制.
- 分析血管平衡和疾病状态期间的EC增殖.
主要方法:
- 使用双重重组合系统 (Cre-loxP和Dre-rox) 来记录时间EC扩散.
- 通过单细胞RNA测序和基因淘汰进行综合遗传追踪.
- 应用这些方法在各种条件下研究小鼠动脉中的EC产生.
主要成果:
- 在成年小鼠的大动脉中量化每月EC增殖率 (约. 3%) 在平衡状态期间.
- 通过Piezo1调节的血流平行地确定了EC分割方向.
- 发现了四个异构的EC亚群,其中EC群1显示出高增殖活性和干细胞标志物丰富.
- 观察到高血压中EC扩散的增加和2型糖尿病中EC扩散的减少,与EC集群1活动相关.
- 发现Hippo和VEGFR2信号通路在大型血管中调节EC增殖的作用.
结论:
- 开发了一种定量方法来追踪大型船舶的EC发电和营业额动态.
- 在大动脉中确定了具有显著的增殖能力的特定EC亚群,这是血管修复策略的潜在目标.
更多相关视频
12:28Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies
Published on: June 3, 2021
5.0K
08:41Assessment of Vascular Tone Responsiveness using Isolated Mesenteric Arteries with a Focus on Modulation by Perivascular Adipose Tissues
Published on: June 3, 2019
9.9K
相关概念视频
Regulation of Angiogenesis and Blood Supply
2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Overview of the Vascular System
2.8K
The vascular system comprises an extensive network of arteries, capillaries, and veins. The vascular system can be broadly divided into the blood and lymphatic systems. Typically, blood vessels can be categorized into three histological regions: tunica intima, tunica media, and tunica adventitia. The tunica intima consists of a single layer of endothelial cells attached to the basal lamina. Underlying the basal lamina is a connective tissue layer and an elastic lamina that gives stability and...
2.8K
Structure of Blood Vessels
4.8K
Blood is circulated throughout the human body through a network of blood vessels called the circulatory system. This system includes arteries that transport blood from the heart to various body parts. These arterial pathways divide into smaller vessels until they reach the arterioles, which further split into capillaries. It is within these minuscule capillaries that the exchange of nutrients and waste products takes place. After this exchange, the blood is collected by venules, which fuse to...
4.8K
Mechanism of Angiogenesis
5.5K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.5K
Autoregulation of Blood Flow
2.3K
Autoregulation mechanisms are characterized by their inherent capacity for self-regulation without necessitating specific nervous stimulation or endocrine control. These mechanisms facilitate the adjustment of blood flow and, therefore, perfusion specific to each tissue region. This self-regulation encompasses chemical signals and myogenic controls.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation....
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation....
2.3K
Blood Flow
69.8K
Blood is pumped by the heart into the aorta, the largest artery in the body, and then into increasingly smaller arteries, arterioles, and capillaries. The velocity of blood flow decreases with increased cross-sectional blood vessel area. As blood returns to the heart through venules and veins, its velocity increases. The movement of blood is encouraged by smooth muscle in the vessel walls, the movement of skeletal muscle surrounding the vessels, and one-way valves that prevent backflow.
69.8K
