LncRNA PSMB8-AS1 刺激血管炎症以加剧动脉样硬化
Shu Li1, Run-Chao He1, Shao-Guo Wu2
1Department of Clinical Laboratory, Guangzhou Women & Children Medical Center, Guangzhou Medical University, Guangdong, China (S.L., R.-C.H., Y.S., K.-L.Z., M.-L.T., T.Z., M.J., X.-H.D., J.W., Y.-W.H.).
Circulation research
|December 12, 2023
概括
长非编码RNAPSMB8-AS1通过增加血管炎症促进动脉样硬化. 抑制PSMB8-AS1或其下游点可能为心血管疾病提供新的治疗策略.
科学领域:
- 分子生物学分子生物学
- 心血管生物学 心血管生物学
- 在RNA生物学,RNA生物学.
背景情况:
- 长非编码RNAs (lncRNAs) 越来越多地被认为是它们在血管生物学和疾病中的作用.
- PSMB8-AS1,一种涉及瘤的lncRNA,在诸如动脉样硬化等心血管疾病中没有明确的作用.
研究的目的:
- 为了研究PSMB8-AS1对血管炎症的影响.
- 确定PSMB8-AS1在动脉样硬化开始和进展中的作用.
主要方法:
- 产生了PSMB8-AS1敲击剂和Apoe敲击剂小鼠,以及Apoe/Psmb9双敲击剂小鼠.
- 给小鼠食用西方饮食12周,诱导动脉样硬化.
- 在体外对内皮细胞进行的功能增加和丧失研究.
主要成果:
- 在人类动脉样硬化斑块中,PSMB8-AS1的水平升高.
- 在Apoe小鼠中,PSMB8-AS1加剧了动脉样硬化,斑块脆弱性和血管炎症.
- 通过NONO/PSMB9/ZEB1通路,PSMB8-AS1对VCAM1和ICAM1进行了上调,促进了单细胞的粘附.
- Psmb9缺陷减轻了PSMB8-AS1诱导的动脉样硬化和炎症.
结论:
- PSMB8-AS1通过NONO/PSMB9/ZEB1轴促进血管炎症和动脉样硬化.
- 向PSMB8-AS1为动脉样硬化心血管疾病提供了潜在的治疗策略.
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