激发性血统分化的特征和Reeler新皮质中的发育保护
Huan-Huan Deng1, Shi-Yuan Tong1, Dan Shen1
1Jing'an District Central Hospital of Shanghai, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institutes of Brain Science, Fudan University, Shanghai, China.
Cell proliferation
|December 12, 2023
概括
大多数新皮质投射神经元通过中间原生细胞 (IPC) 间接产生,而不是直接来自辐射质细胞 (RGC). 这种对大脑发育至关重要的间接神经生成途径,以前由于IPC衍生的神经元亡,被低估了.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
背景情况:
- 新皮层投射神经元主要是从放射性质细胞 (RGCs) 通过中间原生细胞 (IPCs) 间接产生.
- 尽管IPCs在哺乳动物新皮层扩张中的进化意义,但IPCs和RGC分化动态的确切贡献仍未完全理解.
研究的目的:
- 精确量化来自RGCs的直接与间接神经生成的比率.
- 阐明RGC和IPC在新皮层发育过程中的血统动态和差异化特征.
- 调查亡在IPC衍生的神经元贡献中的作用,并比较野生型和Reeler小鼠的神经发生.
主要方法:
- 使用逆转录病毒稀疏标签与碳氧化二酸糖胺 (CFSE) 和Tbr2-CreER菌株相结合.
- 在现场以高时间,空间和细胞分辨率重建单个RGC血统树.
- 在发育中的新皮质中分析了细胞增殖,分化,细胞亡和基因表达 (Cux2).
主要成果:
- 来自RGC的直接神经发生是最小的 (1.90%),而通过IPC的间接神经发生占RGC贡献的79.33%.
- 大约25%的IPC衍生神经元经历了亡,导致对间接神经发生的低估.
- 第一代IPC表现出自我更新,最终产生了四个神经元,并表现出中介祖先的高Cux2水平.
结论:
- 通过IPC间接的神经发生是小鼠中新皮质投射神经元产生的主要方式.
- 这项研究揭示了对RGC谱系贡献的更准确量化,并突出了亡对神经元输出的影响.
- 神经生成特征和神经元命运在Reeler小鼠中被保存,这表明保留了发育机制.
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