相关实验视频

Updated: Jul 8, 2025

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

可编程的适应传感器用于调节CRISPR/Cas12a活动

Yao Yu¹, Qiaoyu Li^2,3, Wen Shi^1,2

¹College of Chemistry & Pharmacy, Northwest A&F University Yangling, Shaanxi 712100, China.

|December 12, 2023

概括

这项研究引入了一种CRISPR介导的可编程口味传感器 (CPAS),用于检测非核酸目标. 该CPAS平台为快速诊断提供了可定制和敏感的方法,用于SARS-CoV-2尖端蛋白和ATP.

科学领域:

分子生物学分子生物学
生物技术是生物技术.
生物感应是一种生物感应.

背景情况:

以CRISPR为媒介的口感传感器越来越多地用于非核酸目标检测.
现有的设计需要复杂的修改,以提高性能和更广泛的应用.

研究的目的:

开发一个多功能且易于定制的CRISPR介导可编程食感器 (CPAS) 平台.
为了提高信号与噪声的比率,通用性和适用传感器的检测范围.

主要方法:

一个CPAS平台被设计使用aptamer,储物库DNA和crRNA识别区域在一个针头结构.
使用T4DNA聚合酶和Cas12a酶,在目标结合时产生光信号.
该平台的定制是通过改变aptamer和locker DNA序列来实现的.

主要成果:

该CPAS平台展示了可调节的锁定器DNA长度用于不同的目标 (S蛋白为7nT,ATP为4nT).
在检测SARS-CoV-2尖峰 (S) 蛋白和腺三酸盐 (ATP) 方面获得了高灵敏度.
与侧向流量试验的整合允许在1小时内进行敏感检测.

结论:

该CPAS平台提供了一个高度可定制和敏感的方法来检测非核酸目标.
它的设计促进了快速,便携式诊断,在各种领域都有潜在的应用.
该平台在临床检测和分子诊断方面表现有前途.

关键词:

这就是CRISPR/Cas12a.它们是Aptamers.生物传感器生物传感器分子识别分子识别在医疗保健中心进行检测.

更多相关视频

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...