通过基于尿解的微生物诱导的碳酸盐沉在土中的Cd固定机制:强调共存的阴离子和元转录组分析
Jiejie Yang1, Luhua Jiang1, Ziwen Guo1
1School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China; Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha 410083, China.
Journal of hazardous materials
|December 12, 2023
概括
微生物诱导碳酸盐沉 (MICP) 通过改变其化学形式,有效地使 (Cd) 在田土壤中停滞不前. 这一过程涉及特定的基因表达和代谢途径,增强土壤微生物对Cd的抵抗力.
科学领域:
- 环境微生物学 环境微生物学
- 土壤科学 土壤科学
- 生物地质化学生物地质化学
背景情况:
- 众所周知,微生物诱导碳酸盐沉 (MICP) 可以使金属固定,从而降低其生物可用性.
- 在大米土壤中 (Cd) 固定化的机制,特别是关于土壤和微生物基因表达的机制,仍然未被充分探索.
研究的目的:
- 通过使用MICP,研究Cd在土中的固定机制.
- 分析Cd的分离转化和MICP期间触发的基因表达和代谢途径.
主要方法:
- 进行了微观宇宙实验,以模拟米土中的MICP.
- 分析了Cd的微分化转换.
- 用转基因组分析来研究基因表达和代谢途径.
主要成果:
- 在330天后,生物可用的Cd显著下降,从0.62到0.29毫克/公斤.
- 由于碳酸盐结合,Fe-Mn氧化物结合和剩余的Cd分数增加,导致了Cd的固定.
- 观察到尿酶基因的上调调节,氨酸生物合成,纯氨酸代谢和氨酸降解途径,以及增强的细菌化学反应,鞭毛组合,甘氨酸生物合成和Cd分泌 (cadA基因).
结论:
- MICP通过各种机制,包括沉物形成,复杂化和吸附,有效地将Cd固定在米土壤中.
- 基于尿溶解的MICP影响特定的微生物代谢途径和基因表达,增强土壤微生物群对Cd的抵抗力.
- 这项研究提供了通过MICP在米土壤中Cd固定化的关键见解.
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