增塑剂乙三甲基酸盐 (ATEC) 诱导脂肪生成和肥胖
Peiqi Li1, Qian Xu1, Weigao Zhang1
1School of Environmental and Biological Engineering, Nanjing University of Science & Technology, 200 Xiaolingwei Street, Nanjing 210094, China.
Toxicology and applied pharmacology
|December 12, 2023
概括
乙三乙酸盐 (ATEC) 是一种常见的塑化剂,可能会促进肥胖和脂肪肝. 在小鼠中,低剂量ATEC暴露增加了体重,脂肪和肝脂,突出了潜在的健康风险.
科学领域:
- 环境健康 环境健康
- 毒理学 毒理学 毒理学
- 代谢障碍 代谢障碍 代谢障碍
背景情况:
- 流行病学研究将塑化剂等环境化学物质与肥胖联系起来.
- 乙三乙酸盐 (ATEC) 是一种非酸盐增塑剂,用于制药和食品包装.
- 由于ATEC的高泄漏率,需要对低环境剂量的健康风险进行评估.
研究的目的:
- 调查ATEC在环境相关的低剂量下可能产生的肥胖和脂肪肝诱导作用.
- 在小鼠模型中评估ATEC暴露对身体组成和肝脏健康的影响.
- 阐明ATEC诱导的脂质积累背后的分子机制.
主要方法:
- 雄性C57BL/6J小鼠在12周内暴露在ATEC (0.1或10μg/kg/天) 中.
- 评估了体重,身体脂肪百分比和肝脏组织学.
- 肝细胞 (老鼠和人类) 用ATEC处理,以测量细胞内脂质积累.
- 基因表达分析确定了参与脂质生成和脂质吸收的途径.
- 使用酶抑制剂和siRNA. stearoyl-coenzyme A脱酶1 (SCD1) 功能进行了研究.
主要成果:
- 在小鼠中,ATEC暴露导致体重增加,体脂百分比增加和脂肪细胞大小增加.
- 在接受ATEC治疗的小鼠和人类肝细胞中观察到良性肝细胞和细胞内脂质负荷增加.
- 在ATEC上调调节了与新型脂质生成和脂质吸收相关的基因.
- SCD1被确定为ATEC诱导的细胞内脂质积累的关键调解者.
结论:
- 这项研究提供了初步证据,证明ATEC的肥胖和脂肪肝诱导作用在低剂量与人类暴露相关.
- ATEC可能会作为环境肥胖原体,因此需要进一步研究其对人类健康的影响.
- 在ATEC暴露引起的代谢干扰中,SCD1起着至关重要的作用.
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