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早期发育中的pepsinogen的表达模式和细胞局部化,并由斑点刀 (Oplegnathus punctatus) 的不同饮食诱导

Yanduo Wu¹, Yongshuang Xiao², Xiao Chen³

¹CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; University of Chinese Academy of Sciences, Beijing, China.

|December 12, 2023

概括

这项研究研究了斑点的刀胃发育和pepsinogen基因表达,以减少幼虫死亡率. 结果揭示了关键的发育阶段和基因表达模式,对于优化水产养殖料策略至关重要.

科学领域:

水产养殖是水产养殖的一种方式.
发展生物学发展生物学
鱼类生理学鱼类生理学

背景情况:

在早期阶段的Oplegnathus punctatus幼虫中,高死亡率与料转化挑战有关.
了解胃发育和消化酶功能对于改善幼虫生存至关重要.

研究的目的:

为了研究Oplegnathus punctatus幼虫胃组织的结构和功能特征.
在幼虫发育过程中识别和表征pepsinogen基因 (OpPGA1和OpPGA2) 以及它们的表达模式.
为了将基因表达和素活性与不同的养策略相关联.

主要方法:

胃组织发育的组织学分析.
定量PCR (qPCR) 用于评估OpPGA1和OpPGA2的基因表达.
素活性测试. 素活性测试.
在现场杂交以定位基因表达.
在各种饮食条件下的基因表达的分析.

主要成果:

胃腺原始体出现在化后17天 (dph),全胃发育在26-35dph.
确定了两个pepsinogen基因,OpPGA1和OpPGA2;它们的表达和pepsin活性在30dph时达到峰值.
在基本/硬饮食中,OpPGA1的表达高,而在植物食饮食中,OpPGA2的表达高.
这两种基因都在胃腺细胞中表达,饮食影响活性.

结论:

这项研究阐明了Oplegnathus punctatus胃生理学的关键发展里程碑.
素原基因表达和活性受到发育阶段和饮食的显著影响.
这些发现为制定有针对性的养策略提供了基础,以提高水产养殖中的幼虫生存率.

关键词:

细胞局部化细胞局部化基因表达基因表达基因表达历史学历史学历史学这种植物是Oplegnathus punctatus.素的活性为素.这种基因是Pepsinogen基因.

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