在意大利检测到的与Streptococcus ruminantium相关的绵羊乳腺炎疫情与牛分离物不同
Maria Nives Rosa1, Ben Vezina2, Gavino Marogna1
1Istituto Zooprofilattico Sperimentale della Sardegna "G. Pegreffi", 07100, Sassari, Italy.
Veterinary research
|December 13, 2023
概括
这项研究详细介绍了在意大利由Streptococcus ruminantium引起的严重绵羊乳腺炎疫情. 基因组分析揭示了克隆传播和独特的绵羊和牛S.动物血统,突出了病原体的机会性质.
科学领域:
- 兽医微生物学 兽医微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 杂杆菌 (Streptococcus ruminantium) 导致牛和绵羊的疾病,但很少报告疫情.
- 整个基因组测序很少用于识别S. ruminantium.
- 这项研究解决了在绵羊中罕见的S. ruminantium疫情.
研究的目的:
- 报告意大利第一个由S. ruminantium引起的严重绵羊乳腺炎疫情.
- 通过基因组鉴定疫情孤立的特征,并将其与历史和全球的S. ruminantium菌株进行比较.
- 为了评估抗菌素敏感性和划分S. ruminantium种群结构.
主要方法:
- 从受影响的母羊中分离和识别S. ruminantium.
- 疫情和意大利历史孤立的全基因组测序.
- 与公开可用的S. ruminantium基因组进行比较基因组分析.
- 抗生素敏感性测试 (16种抗生素的MIC).
- 单核酸变异 (SNV) 分析疫情相关性和人口结构.
主要成果:
- 在意大利 (2022年) 一次严重的绵羊乳腺炎疫情被归因于S. ruminantium.
- 基因组分析证实了10只母羊的克隆性爆发,其中两只被不同的血统感染.
- 历史意大利的隔离品在遗传上与爆发菌株有很大距离.
- 绵羊和牛的S. ruminantium分离物形成了不同的单类群.
- 所有分离物对测试的抗微生物药物敏感,除了kanamycin.
结论:
- 基因组监测发现了多种S. ruminantium血统,涉及到绵羊乳腺炎疫情.
- 绵羊和牛的分离物代表了基因上不同的种群.
- 这些发现支持S. ruminantium的机会性病原体.
- 基因组监测对于检测疫情和了解这种多宿主病原体至关重要.
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