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Updated: Jul 8, 2025

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes

Published on: September 17, 2012

检查细胞特异定位在有光的Aedes aegypti组织中在现场混合化

Farwa Sajadi¹, Jean-Paul V Paluzzi²

¹Department of Biology, York University, Toronto, Ontario M3J 1P3, Canada.

Cold Spring Harbor protocols

|December 13, 2023

概括

光在位杂交 (FISH) 可视化蚊子肠道组织中的基因位置. 这种方法使用RNA探针和信号放大用于精确的受体转录映射.

科学领域:

分子生物学分子生物学
基因组学就是基因组学.
昆虫学昆虫学是一门学科.

背景情况:

光 in situ 杂交 (FISH) 是可视化细胞和组织内核酸序列的关键技术.
在各种动物模型中,FISH可实现基因映射和基因组可视化.
了解转录本地化对于研究基因功能和生物生物学至关重要.

研究的目的:

适应和应用FISH技术,以在Aedes aegypti蚊子的肠道组织中定位受体转录.
详细说明合成RNA探针和在蚊子肠道样本上进行FISH的方法.
为了证明提拉胺信号放大用于增强FISH检测的实用性.

主要方法:

合成标有光体或滴氧基因的RNA探针.
用合成的RNA探针化固定*Aedes aegypti*肠道组织/器官.
提拉胺信号放大剂的应用,以提高信号检测,以改善可视化.

主要成果:

在Aedes aegypti*的肠道组织中成功定位了特定的受体转录.
演示了开发的FISH协议在蚊子中转录映射的有效性.
通过提拉胺信号放大实现了增强的信号检测.

结论:

鱼是一种强大的工具,用于绘制基因和转录本地化在 * Aedes aegypti * 蚊子肠道组织.
描述的方法为研究受体转录分布提供了一个强大的协议.
泰拉米德信号放大显著提高了这种模型系统中FISH分析的灵敏度.

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Published on: June 28, 2012

Fluorescence in situ Hybridizations FISH for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Fluorescence in situ Hybridizations FISH for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Published on: February 24, 2014

相关概念视频

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...