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Updated: Jul 8, 2025

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乳酸盐-乳酸化-HSPA6轴通过损害IFN-β的产生促进PRRSV的复制

Yu Pang¹, Yanrong Zhou², Yucheng Wang²

¹National Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

Journal of virology

|December 13, 2023

概括

在猪生殖和呼吸系统综合征病毒 (PRRSV) 感染期间乳酸水平升高会诱导乳化,通过上调HSPA6和抑制干扰素β生产来促进病毒生长. 这揭示了PRRSV的新治疗点.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
病毒学病毒学
免疫学免疫学免疫学

背景情况:

乳酸是表观遗传调节剂,诱导乳糖化,影响基因表达.
猪生殖和呼吸系统综合征病毒 (PRRSV) 提高乳酸水平,但其在感染中的作用尚不清楚.
病毒诱导的乳在病毒感染中的重要性在很大程度上是未被探索的.

研究的目的:

为了研究乳酸和乳糖在PRRSV感染中的作用.
为了识别在PRRSV感染期间通过乳糖化调节的下游基因.
阐明乳化影响病毒增殖的机制.

主要方法:

在受PRRSV感染的细胞中与RNA测序 (RNA-seq) 相结合的目标和标记 (CUT&Tag) 下的切割.
剂量反应实验,以评估PRRSV诱导的乳化.
后续实验验证基因功能和蛋白质相互作用.

主要成果:

乳酸对于PRRSV的扩散是必不可少的,而PRRSV感染会增加细胞乳化.
由PRRSV诱导的乳化可调节热冲击70kDa蛋白6 (HSPA6) 的表达.
HSPA6通过破坏TRAF3-IKKε复合体的形成来抑制干扰素 (IFN) -β的产生,促进病毒生长.

结论:

乳酸-乳酸化-HSPA6通路通过抑制IFN-β诱导促进PRRSV复制.
针对这种途径为PRRSV控制提供了潜在的治疗策略.
这项研究建立了乳酸和病毒生命周期之间的联系,进步了对病毒感染中的表观遗传调节的理解.

关键词:

热冲击 70 kDa 蛋白质6 (HSPA6) 的热冲击干扰子干扰子干扰子乳酸乳酸是一种乳酸.乳化乳化乳化猪生殖和呼吸系统综合征病毒

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