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使用KinExA和表面等离子体共振确定高亲和抗体的亲和力

M Frank Erasmus¹, Molly Dovner², Fortunato Ferrara¹

¹Specifica, LLC, a Q2 Solutions Company, Santa Fe, NM, USA.

|December 13, 2023

概括

通过比较两个抗体亲和度测量技术,Carterra LSA和动力排除试验 (KinExA),发现KinExA为高亲和度抗体提供了更好的性能. 单点KineXA查提高了对强效抗体克隆的排名吞吐量.

科学领域:

生物物理学的生物物理.
免疫学免疫学免疫学
药物发现药物发现药物发现

背景情况:

治疗性单克隆抗体是一个快速扩张的药物类别,需要精确的生物物理特征.
表面等离子体共振 (SPR) 是一种常见的抗体亲和度测量方法,但它与非常高亲和度的分子 (从皮科莫拉到女性分子) 斗争.

研究的目的:

为了比较基于SPR的卡特拉LSA和动力排除试验 (KinExA) 测量抗体亲和力的性能.
评估这些技术对抗SARS-CoV-2受体结合域的高亲和抗体的适用性.

主要方法:

使用Carterra LSA和KinExA.测量48种针对SARS-CoV-2受体结合域的抗体的亲缘关系.
两种技术之间的动态范围,吞吐量和精度的比较分析.
使用KinExA.A.进行单点查的演示.

主要成果:

通过LSA和KineXA测量的亲和关系之间观察到54%的对应.
当观察到两倍或更大的差异时,Kinexa通常报告了更紧密的亲和力.
单点KineExA选与平衡测量保持了强烈的相关性,并改善了吞吐量.

结论:

使用体外图书馆平台,可以有效地产生高亲和度抗体.
KinExA在测量非常高亲和力的抗体方面比LSA具有优势,并通过单点查提供了更好的吞吐量.
KinExA能够准确排序具有异常紧密的结合性质的强效抗体克隆.

关键词:

抗体是对抗体的一种.抗体亲和力抗体亲和力抗体的特征表征.抗体治疗药物抗体治疗药物发现药物的发现.动力排斥测试试验动力排斥测试试验表面的等离子体共振.

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