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在ESRF的TR-icOS设置:在蛋白质晶体上的微秒时间分辨率UV-Vis吸收光谱

Sylvain Engilberge¹, Nicolas Caramello², Sergei Bukhdruker²

¹Université Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale (IBS), 71 Avenue des Martyrs, CS 10090, 38044 Grenoble CEDEX 9, France.

Acta crystallographica. Section D, Structural biology

|December 13, 2023

概括

时间分辨率宏分子晶体学 (TR-MX) 使用新的同步子束线进行光活性蛋白的微秒研究. 一种新型仪器,TR-icOS,使单晶的光谱分析能够优化TR-MX实验.

科学领域:

结构生物学结构生物学
频谱学是一种光谱学.
生物物理学的生物物理.

背景情况:

时间分辨率宏分子晶体学 (TR-MX) 正在推进专门的同步子束线.
现在可以对光活性蛋白进行微秒时间解析的机械学研究.
在蛋白质晶体上进行时间解析的光谱实验对于确定最佳时间延迟至关重要.

研究的目的:

开发和验证单个蛋白质晶体的时间解析光谱分析的设置.
建立时间解析的宏分子晶体学 (TR-MX) 的实验参数.
在光反应研究中研究激光诱导的工件.

主要方法:

在欧洲同步射线辐射设施的时间解析晶体光谱学 (TR-icOS) 装置的建造.
使用纳秒激光作为和微秒的光闪光灯作为探针.
从单晶中记录探针光谱,时间延迟从微秒到秒.
通过改变激光脉冲能量来执行功率定位,以检测两光子吸收器件.

主要成果:

该TR-icOS设置允许从单晶快速记录探头光谱.
对bacteriorhodopsin的光循环进行了监测,显示激光流动的变化超过100mJ/cm2.
为了成功的TR-MX,确定了激光脉冲能量和延迟的实验参数.

结论:

TR-icOS仪器提供了优化TR-MX实验的重要数据.
了解激光诱导的工件对于精确的光反应机械学研究至关重要.
这种方法促进了使用TR-MX的光活性蛋白和其他系统的研究.

关键词:

在背后的hodopsin.在晶体光学光谱学.具有光活性蛋白质的蛋白质.探针光谱学探针光谱学序列同步子晶体学序列同步子晶体学

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