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用光PCR微针阵列进行空间瘤活检.

Xiaoxuan Zhang1, Guopu Chen1, Yu Wang1

  • 1Department of Rheumatology and Immunology, Nanjing Drum Tower Hospital, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.

Innovation (Cambridge (Mass.))
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概括

一个新的微针阵列 (MNA) 平台使有效的空间瘤活检能够提高癌症诊断. 这种光聚合酶链反应 (F-PCR) MNA平台简化了在各种样本中检测瘤生物标志物的过程.

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科学领域:

  • 生物技术是生物技术.
  • 材料科学 材料科学 材料科学
  • 在瘤学瘤学.

背景情况:

  • 活检对于准确的瘤诊断至关重要,为瘤发生和进展提供了详细的见解.
  • 目前的活检方法可能具有侵入性,并且可能并不总是有效地捕捉空间异质性.

研究的目的:

  • 开发一个高效的空间瘤活检平台,使用微针阵列 (MNA) 进行光聚合酶链反应 (F-PCR).
  • 为了使瘤核酸生物标志物的快速和立体采集,用于癌症标志物的发现.

主要方法:

  • 使用自下自上自组装和自上自下光刻法制造MNA.
  • 使用一种疏水性氧化纳米颗粒组装基板和石墨烯气凝-水凝混合微针峰.
  • 采用离散的可湿性,以实现高效的流体管理和在微针尖上直接进行F-PCR反应.

主要成果:

  • F-PCR-MNA平台展示了高效的空间瘤活检和核酸生物标志物的立体收集.
  • 在标准溶液,小鼠组织和临床标本中成功检测了肺癌的DNA生物标志物.
  • 该平台的离散可湿性使组织和PCR液体易于去除,每个微针作为一个独立的反应场所.

结论:

  • 开发的F-PCR-MNA平台为瘤活检提供了一种实用且创新的方法.
  • 这项技术在推进瘤诊断和生物标志物发现方面具有重大潜力.
  • 该MNA系统为检测癌症DNA生物标记物提供了一种有效的方法,具有潜在的临床应用.