CTSS非同名突变对子大小在北马山羊的影响
Yuan Zhang1,2,3, Xiang Chen1,2,3, Yong Ruan1,2,3
1Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang, China.
Frontiers in veterinary science
|December 13, 2023
概括
凯瑟普辛S (CTSS) 基因变异显著影响山羊子大小. 特定的CTSS突变改变了蛋白质的结构和功能,提供了对生殖特征和山羊品种演变的见解.
科学领域:
- 动物遗传学和动物繁殖
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
背景情况:
- 素S (CTSS) 是一种囊蛋白酶,涉及到山羊的生殖调节.
- CTSS对山羊子大小的确切影响需要进一步阐明.
- 了解CTSS基因多态性对于改善山羊的繁殖性能至关重要.
研究的目的:
- 为了研究CTSS基因多态和子大小在北马山羊之间的关系.
- 用生物信息学分析非同义CTSS突变对蛋白质结构和功能的影响.
- 为了提供CTSS在山羊子大小的监管机制的理论基础.
主要方法:
- 在山羊的淋巴腺轴中分析CTSSDNA序列和mRNA表达.
- 在CTSS基因中单核酸多态 (SNPs) 的识别和基因定型.
- 对非同名突变对CTSS蛋白质结构和保存的影响的生物信息评估.
主要成果:
- 在淋巴腺轴上,CTSS表达有所不同,多羊群的子宫表达高,单羊群的输卵管表达高.
- 四个CTSS SNPs (g.7413C→T,g.8816A→T,g.9191T→G,g.10193G→A) 与幼大小 (p<0.05) 有显著的相关性.
- 非同义基因突变p.S161F和p.N219Y改变了CTSS蛋白质的形状,稳定性和功能,在不同物种中保持不同.
结论:
- 在北马山羊中,CTSS基因多态性与子大小有显著的关联.
- 鉴定的CTSS突变影响蛋白质结构和功能,影响生殖特征.
- 这些发现有助于理解导致山羊子大小变化的遗传机制.
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