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黄素减轻了睡眠剥夺对大鼠下丘脑侧腔室核的影响
Mahboobeh Erfanizadeh1, Ali Noorafshan1,2, Mohammad Reza Namavar1,3
1Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
IBRO neuroscience reports
|December 13, 2023
概括
慢性REM睡眠剥夺会损害下丘脑的副腹腔核 (PVN). 黄素治疗保护了这种损伤,保留了PVN体积和神经元排列.
科学领域:
- 神经科学是一个神经科学.
- 时间生物学 时间生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 下丘脑 (PVH) 的副腹核对于调节昼夜节律和应激反应至关重要.
- 根据假设,慢性REM睡眠剥夺 (CSD) 会在PVH内引起损伤.
- 了解黄素等化合物对睡眠剥夺引起的神经变化的神经保护作用很重要.
研究的目的:
- 研究CSD对雄性大鼠PVH结构完整性和细胞排列的影响.
- 评估黄素在PVH中对CSD诱导的变化的潜在抗亡和神经保护作用.
主要方法:
- 在21天的时间里,老鼠使用修改后的多平台装置接受了CSD.
- 黄素 (100 mg/kg/day) 或载体 (PBS) 被口服给不同的实验组.
- 采用立体学方法,沃罗诺伊模块化和TUNEL测试来评估PVH体积,细胞数量,空间神经元分布和细胞亡.
主要成果:
- CSD导致PVH体积和总细胞数量的显著减少,伴随着死亡神经元的增加.
- 在PVH中,帕沃细胞和大细胞神经元的空间分布在CSD后从常规转变为随机.
- 黄素的使用不仅减轻了体积和细胞损失,而且还恢复了PVH神经元的正规空间布局.
结论:
- 脑神经疾病诱导神经元亡,并改变PVH内的神经元的空间组织,导致体积和细胞数量减少.
- 口服黄素显示出显著的神经保护作用,保护PVH结构和功能免受睡眠剥夺引起的损伤.
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