昆虫的多重体G四重体折叠成不同紧度和稳定的反平行结构,在K+和Na+溶液中
Chao Gao1, Jixin Chen1, Naureen Anwar2
1National R&D Center for Se-rich Agricultural Products Processing, Hubei Engineering Research Center for Deep Processing of Green Se-rich Agricultural Products, School of Modern Industry for Selenium Science and Engineering, Wuhan Polytechnic University Wuhan 430023 China wzqsnu@whpu.edu.cn yi.he@whpu.edu.cn.
RSC advances
|December 13, 2023
概括
昆虫端粒G-四重复合体在Na+溶液中更稳定,更紧. 化合物BMPQ-1可选择性地结合昆虫多重体G-四重复合体,并抑制害虫细胞的增殖.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 结构生物学 结构生物学
背景情况:
- 人类端粒形成具有治疗潜力的G-四复合体.
- 昆虫端粒 (TTAGG) 也形成G四复合体,但它们的结构和相互作用的理解较少.
- 已知化合物BMPQ-1可以结合人类的G-四重复合体.
研究的目的:
- 在K+和Na+溶液中比较昆虫单体和多体G四重复的稳定性和紧性.
- 研究BMPQ-1与昆虫多重体G-四重体的相互作用.
- 评估BMPQ-1对害虫细胞的生物活性.
主要方法:
- 循环二元化 (CD) 光谱扫描用于分析G-四重复结构和稳定性.
- 光共振能量转移 (FRET) 来评估结构紧性.
- 结合性测试以确定BMPQ-1的选择性和相互作用模式.
- 细胞增殖测试用于评估生物效应.
主要成果:
- 昆虫端粒G四重复体在K+和Na+溶液中采用反平行结构.
- 在Na+溶液中,G-四复合体显示出明显更高的稳定性和紧性.
- BMPQ-1 对于多聚合的昆虫G-四重复合体 (Bom37) 比单聚合物 (Bom17) 的选择性更强.
- 对于Bom37.37的K+和Na+溶液,BMPQ-1的结合模式不同.
- BMPQ-1显著抑制了害虫细胞的增殖.
结论:
- 昆虫端粒G四重复体在不同的离子环境中表现出独特的结构性质.
- 作为昆虫多重体G-四重体的选择性联体,BMPQ-1显示出有前途,在害虫控制中具有潜在的应用.
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