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两个近红外遗传编码电压指示器的特征

Chenchen Song^1,2, Mikhail E Matlashov³, Daria M Shcherbakova³

¹Imperial College, Laboratory for Neuronal Circuit Dynamics, London, United Kingdom.

|December 13, 2023

概括

评估了新的近红外遗传编码电压指示器 (GEVIs) 用于神经元活动监测. 虽然nirButterfly更明亮,更快,但Archon1提供了更大的信号,在所有参数中都没有卓越.

科学领域:

神经科学是一个神经科学.
在光学成像系统中,光学成像
生物技术是生物技术.

背景情况:

遗传编码的电压指示器 (GEVIs) 对神经元活动的光学成像至关重要.
近红外 (NIR) GEVIs为体内哺乳动物大脑监测提供了优势.
高性能NIR GEVIs的可用性和全面评估仍然有限.

研究的目的:

描述和比较两个近期的NIR GEVIs,Archon1和nirButterfly. 这两种物种的特征.
为研究人员提供支持数据,帮助他们选择适合特定应用的NIR GEVIs.
评估这些GEVI对人口成像的适用性.

主要方法:

在Archon1和nirButterfly的并排表征.
评估亮度,灵敏度和动力学.
在人口成像背景下进行评估.

主要成果:

nirButterfly的亮度比Archon1.1的亮度高出七倍.
在人口成像中,nirButterfly 与 Archon1 相比表现出更快的动力学.
阿尔康1产生的信号幅度大于nirButterfly.

结论:

无论是Archon1还是nirButterfly在所有关键参数 (亮度,动力学,灵敏度) 中都没有普遍优于其他参数.
在Archon1和nirButterfly之间做出选择取决于具体的实验要求.
这种比较性表征为未来使用NIR GEVIs进行体外和体外实验设计提供了宝贵的见解.

关键词:

遗传编码的电压指示器在iRFPFP中,这就是Opsin的选择.电压成像电压成像电压感应领域是电压感应领域.广场光学成像技术的广泛应用.

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