相关实验视频

Updated: Jul 8, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
09:25

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

46.2K

最佳实践:建立和运营一个中等规模的冷EM设施.

Xing Meng1,2, Ishara Ratnayake1,2, Martha L Escobar Galvis3

  • 1David Van Andel Advanced Cryo-Electron Microscopy Suite, Van Andel Institute, Grand Rapids, MI, United States.

Frontiers in molecular biosciences
|December 13, 2023
PubMed
概括

建立一个中等规模的冷电子显微镜 (cryo-EM) 设施需要精心规划,设备选择和IT解决方案. 本指南为优化运营,管理维护和了解高性能单颗粒冷EM的成本提供了洞察力.

关键词:
信息技术工具 信息技术工具核心设施的核心设施.低温电磁波冷却器 (Cryo-EM) 是一个非常好的方法.基础设施基础设施基础设施的基础设施.维护维护维护维护维护维护维护操作操作操作的操作建立一个设置.

更多相关视频

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells11:55

Cryo-Structured Illumination Microscopic Data Collection from Cryogenically Preserved Cells

Published on: May 28, 2021

4.2K
Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy09:16

Manual Blot-and-Plunge Freezing of Biological Specimens for Single-Particle Cryogenic Electron Microscopy

Published on: February 7, 2022

6.4K

相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.3K