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Updated: Jul 8, 2025

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry
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使用实时PCR产品的直接测序进行法医物种识别的实用工作流

Masanori Doi1,2, Toshifumi Nakagawa3, Migiwa Asano4

  • 1Forensic Science Laboratory, Ehime Prefectural Police Headquarters, 2-2 Minamihoribatacho, Matsuyama, Ehime, 790-8573, Japan. doi.bio.rese@gmail.com.

Molecular biology reports
|December 13, 2023
PubMed
概括

这项研究为法医科学引入了一种简化物种识别工作流程,利用实时PCR直接测序在案例工作中进行快速准确的动物DNA分析. 该方法简化了常规测试,并提高了法医实验室的物种识别能力.

关键词:
DNA条形码是指DNA的条形码.法医科学 法医科学 法医科学实时PCR直接测序实时PCR直接测序桑格尔序列是什么意思种类鉴定 种类鉴定

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
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