托克索普拉斯马蛋白酸酶6催化子单元 (TgPP6C) 对于细胞循环进展和毒性至关重要
Qin-Li Liang1, Lan-Bi Nie1, Hany M Elsheikha2
1State Key Laboratory for Animal Disease Control and Prevention, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.
PLoS pathogens
|December 13, 2023
概括
蛋白酸酶6催化子单元 (TgPP6C) 对于Toxoplasma gondii的复制和毒性至关重要. 缺少它会破坏细胞分裂,减弱寄生虫的病原性,为新疗法提供潜在的点.
科学领域:
- 分子寄生虫学 分子寄生虫学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 蛋白酸酶调节关键的Toxoplasma gondii功能,包括增殖和分化.
- 了解这些调节器是控制寄生虫感染的关键.
研究的目的:
- 在Toxoplasma gondii中识别和表征蛋白合酶6 (TgPP6) 子单元.
- 阐明TgPP6子单元在寄生虫的溶解周期,细胞分裂和毒性中的作用.
主要方法:
- 对TgPP6子单位 (TgPP6C,TgPP6R,TgPP6S) 的识别和表征.
- 功能性研究涉及基因删除和对寄生虫生长和复制的评估.
- 蛋白质组分析以确定改变的蛋白质酸化.
- 在小鼠中的病毒性测定和诱导保护性免疫力的评估.
主要成果:
- TgPP6C和TgPP6R构成了对寄生虫生长和复制至关重要的复合体.
- TgPP6C 除严重损害了内分生 (子细胞分裂),导致细胞结构异常.
- TgPP6C对于调节细胞循环蛋白的酸化状态至关重要.
- 删除TgPP6C显著降低了小鼠中的寄生虫毒性.
- 用TgPP6C缺乏的寄生虫进行免疫接种,可以防止后续感染.
结论:
- TgPP6C是Toxoplasma gondii细胞分裂,复制和致病性的关键调节者.
- TgPP6C代表了针对毒素菌的治疗干预的潜在目标.
更多相关视频
11:21Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity
Published on: March 12, 2015
11.1K
11:37QTL Mapping and CRISPR/Cas9 Editing to Identify a Drug Resistance Gene in Toxoplasma gondii
Published on: June 22, 2017
16.3K
相关概念视频
01:42Microtubule Associated Proteins (MAPs)
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
03:03mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
01:24Caspases
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside cells.
01:25cAMP-dependent Protein Kinase Pathways
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
01:28Toxoplasmosis
Toxoplasmosis, a zoonotic disease caused by the protozoan Toxoplasma gondii, poses significant public health challenges globally due to its high seroprevalence and varied clinical manifestations. As an obligate intracellular parasite, T. gondii can infect all warm-blooded vertebrates, but felids are its only definitive hosts, shedding unsporulated oocysts into the environment. Humans typically acquire the infection through ingestion of tissue cysts in undercooked meat or oocysts from...