评估多溶剂大小排除色谱列,其中包含2μm以下的颗粒,用于合成聚合物的表征
Sensen Shen1, Mubasher Bashir2, Steve Huysman2
1Dow, Analytical Science, 201203 Shanghai, China.
Journal of chromatography. A
|December 13, 2023
概括
新型尺寸排除色谱 (SEC) 列允许使用有机和水性溶剂快速进行聚合物分析. 这些柱子提供更快的分离和准确的分子重量确定合成聚合物,如聚乙烯和聚乙烯甘醇.
科学领域:
- 聚合物化学 聚合物化学
- 分析化学 分析化学
- 染色体学 染色体学 是一种染色学.
背景情况:
- 超高性能液态染色学 (UHPLC) 已与尺寸排除染色学 (SEC) 结合用于先进的聚合物分析.
- 传统的SEC方法往往面临多溶剂兼容性的局限性,限制其用于有机或水性化剂.
研究的目的:
- 为了证明新型SEC列装有1.9微米颗粒的功能,用于合成聚合物表征.
- 评估这些新SEC列对有机和水性化剂的多溶剂兼容性.
主要方法:
- 使用了新的AdvanceBio SEC列,其中有1.9微米颗粒.
- 使用有机和水性移动相进行了聚钢 (PS) 和聚乙烯甘醇 (PEG) 的分离.
- 评估分离速度,峰值形状,可重复性和分子重量确定精度.
主要成果:
- 与标准SEC列相比,PS和PEG的分离速度大约是标准SEC列的六倍,分辨率和峰值形状相似.
- 证明了近乎准确的PEG在四水富兰 (THF) 的分子量确定,这是其他SEC列的共同挑战.
- 在多次在水和THF之间切换溶剂后,保留时间在0.5%以下的相对标准偏差 (RSD%) 确认了出色的可重复性.
结论:
- 新的SEC列为聚合物分析提供了强大而通用的选择,支持有机和水性移动相.
- 这些列可以快速准确地描述合成聚合物,包括分子量和分布.
- 使用探测器进行进一步的连线,可以提供全面的了解聚合物成分,度和结构.
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