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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
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Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in Callinectes sapidus
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通过LC-MS/MS使用MRM方法,探索从毒中获取的来源识别方法.

Lei Zhu1, Junde Xu1, Rui Gan1

  • 1Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and Jiangsu Key Laboratory for High Technology Research of TCM Formulae, College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.

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概括
此摘要是机器生成的。

这项研究引入了毒的,作为传统中医药质量评估的新方法. 研究人员开发了定量方法来分析这些,改善了对毒源的评估.

关键词:
这是LC-MS/MS.这是一个MRMM.酸是一种酸.源标识来源的标识青的毒液是青的毒液.

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Synthesis and Structure Determination of &#181;-Conotoxin PIIIA Isomers with Different Disulfide Connectivities
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科学领域:

  • 药理学 药理学是指药理学的学科.
  • 分析化学 分析化学
  • 传统中国医药 传统中国医药

背景情况:

  • 青毒是一种传统中医药 (TCM),其来源和制备方法各异.
  • 现有的质量评估侧重于小分子,忽视了宏分子和蛋白质.
  • 需要实用,定量方法来评估青毒中的宏分子成分.

研究的目的:

  • 探索青毒作为来源识别和质量评估的新方法.
  • 开发和应用定量方法来分析不同青毒源中的.
  • 为未来对青毒的宏分子物质的研究奠定基础.

主要方法:

  • 高性能液体染色学-四极飞行时间质谱 (HPLC-Q-TOF) 用于的识别.
  • 高性能液体染色学-四极-线性离子陷质谱 (HPLC-Q-Trap) 与多重反应监测 (MRM) 用于量化.
  • 部分最小平方区分分析 (PLS-DA) 用于数据分析和源评估.

主要成果:

  • 使用质谱学成功识别了毒水提取物中的.
  • 在各种青毒源中定量分析了.
  • 证明了形状分析和PLS-DA用于评估毒素质量和来源的有效性.

结论:

  • 体是识别和评估青毒素质量的有价值标记物.
  • 开发的定量分析方法为TCM质量控制提供了实用方法.
  • 这项研究强调了研究天然产品中的宏分子成分的重要性.