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SMYD3激活TCA循环,促进巨细胞中的M1-M2转化

Wenqiang Zhu¹, Lina Liu², Jinjing Wu²

¹Department of Pathology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi Province, China.

International immunopharmacology

|December 13, 2023

概括

基因组甲基转移酶SMYD3通过激活TCA循环,驱动巨细胞M1到M2的偏离. 它通过H3K4me3调节新陈代谢酶,促进ROS生成和表型切换的线粒细胞吸收.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
细胞生物学细胞生物学
代谢过程中的代谢.

背景情况:

SMYD3是一种基因组甲基转移酶,通过H3K4me3.3调节基因转录.
表观遗传修饰对于巨细胞两极分化至关重要.
在巨细胞极化中的SMYD3的作用仍然在很大程度上是未知的.

研究的目的:

研究SMYD3对巨细胞两极分化和表型切换的影响.
阐明SMYD3影响巨细胞表型的分子机制.

主要方法:

RNA测序 (RNA-seq) 用于分析基因表达变化.
染色体免疫沉降测序 (ChIP-seq) 用于识别SMYD3向基因.
使用LPS/IFN-γ对巨细胞进行刺激,以诱导M1极化并观察M1-M2转化.

主要成果:

在晚期的M1到M2巨细胞两极分化中,SMYD3起着关键作用.
在M1-M2转化过程中,SMYD3激活了巨细胞中的三碳酸 (TCA) 循环.
SMYD3通过H3K4me3向代谢酶 (CS,SDHC,PC),增加酸盐和减少糖酸盐.
SMYD3促进ROS生成和线粒体复杂基因表达,诱导线粒体和M1-M2切换.

结论:

SMYD3促进了M1到M2巨细胞的表型过渡.
SMYD3通过激活TCA循环来促进这种过渡.
这种激活涉及通过H3K4me3.3.通过代谢酶转录的同时调节.

关键词:

基因组胺基因的修改巨细胞的表型转换转换.线粒细胞衰变 - - 线粒细胞衰变 (mitophagy) 是一种在SMYD3中,在TCA循环中,TCA循环是

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