使用胆离子液体调节基于植物的脂质纳米颗粒的结构多态和蛋白质冠状组成
Mohamad El Mohamad1, Qi Han1, Andrew J Clulow2
1School of Science, STEM College, RMIT University, 124 La Trobe Street, Melbourne, VIC 3000, Australia.
Journal of colloid and interface science
|December 13, 2023
概括
离子液体增强了基于脂质的液晶晶纳米粒子 (LCNP) 的稳定性. 特定的离子液体及其离子会影响人类血清中的LCNP结构和蛋白质冠状形成.
科学领域:
- 纳米技术纳米技术
- 材料科学 材料科学 材料科学
- 生物化学 生物化学
背景情况:
- 基于脂质的液晶液态纳米粒子 (LCNPs) 显示出临床应用的有前途潜力.
- 当暴露在生物液体中时,LCNPs经常面临稳定性问题,这阻碍了它们的翻译.
- 离子液体 (ILs) 正在作为添加剂来改善LCNP的稳定性和结构完整性.
研究的目的:
- 研究各种基于胆的离子液体 (IL) 对基于植物醇 (PHY) 的LCNP的结构稳定性的影响.
- 分析不同ILs在人血清中化LCNPs的相位行为和蛋白质冠状形成.
- 了解IL结构,LCNP内部组织和生物流体相互作用之间的关系.
主要方法:
- 使用21种基于胆的离子液体 (IL) 的库,制备基于植物 (PHY) 的LCNP,具有多种离子.
- 使用小角度X射线散射 (SAXS) 描述LCNP结构和相位行为.
- 在人血清中化LCNPs,并通过液体染色学-质谱学 (LC-MS) 和蛋白质学分析冠状蛋白组成.
主要成果:
- 萨克斯分析显示,IL度和离子结构显著影响LCNP阶段行为.
- 在人血清中化诱导了从立方 (Q2) 到六角 (H2) 中相过渡,由特定的IL调节.
- 蛋白质组学在LCNP蛋白冠状体中确定了像专辑蛋白和阿波利波蛋白这样的关键蛋白质,其组成因IL而有所不同.
结论:
- 基于胆的IL可以有效地稳定PHY-LCNP,并调节它们对生物流体的结构反应.
- 选择IL离子对于确定LCNP相变和血清中的蛋白相互作用至关重要.
- 了解IL-LCNP-蛋白冠状体相互作用对于基于LCNP的治疗方法的成功临床转化至关重要.
关键词:
氨基酸是氨基酸的组成部分.胆离子液体 胆离子液体立方体体是一个立方体体.六合体体是指六合体的组成部分.人类血清的人类血清脂质纳米颗粒的使用方法利约特罗普液晶是一种液晶.植物利醇是一种植物利醇.蛋白质冠状冠状病毒更多相关视频
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