在蓝果成熟过程中调节胡卜素代谢和ABA生物合成
Xiaobai Li1, Dandan Zhang2, Xuhao Pan3
1Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, China; Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants, Zhejiang Normal University, Jinhua 321004, China.
Plant physiology and biochemistry : PPB
|December 13, 2023
概括
蓝果的成熟涉及降低胡卜素生物合成和增强新陈代谢,以乙烯加速这些变化. 由于上调的VcNCED1/3,可能通过ZEP独立的途径积累了酸 (ABA).
科学领域:
- 植物生理学 植物生理学
- 生物化学 生物化学
- 果实成熟 果实成熟
背景情况:
- 胡卜素和酸 (ABA) 是重要的植物化合物,但它们在蓝 (Vaccinium corymbosum) 水果中的新陈代谢知之甚少.
- 了解胡卜素和ABA通路对于水果的质量和发育至关重要.
研究的目的:
- 为了阐明蓝果成熟期间的胡卜素和ABA代谢.
- 研究VcCCD1和VcNCED1/3在这些过程中的作用.
- 研究乙烯 (乙烯和1-MCP) 对胡卜素和ABA生物合成的影响.
主要方法:
- 液体染色学-质谱学 (LC-MS) 用于胡卜素和ABA分析.
- 生物合成途径的蛋白质组分析.
- 在体外和体外酶活性测定VcCCD1和VcNCED1/3.3.
- 定量PCR (qPCR) 用于评估基因表达变化.
主要成果:
- 胡卜素生物合成减少,而代谢在成熟期间增加,导致胡卜素水平降低.
- VcCCD1积极降解主要的胡卜素,如β-胡卜素和.
- 由于上调的VcNCED1/3,ABA迅速积累,有证据表明ZEP独立的途径.
- 乙烯处理通过加速胡卜素降解和ABA生物合成来模仿自然成熟.
结论:
- VcCCD1和VcNCED1/3是蓝果成熟的关键酶,调节着胡卜素和ABA水平.
- 一个新的ZEP独立的途径可能有助于蓝中的ABA合成.
- 在水果发育过程中,乙烯在调节胡卜素和ABA代谢方面发挥着重要作用.
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