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使用CRISPR库的piggyBac转位子输送进行突变模型的并行工程

Xander Nuttle1, Nicholas D Burt2, Benjamin Currall2

  • 1Center for Genomic Medicine and Department of Neurology, Massachusetts General Hospital, Boston, MA, USA; Department of Neurology, Harvard Medical School, Boston, MA, USA; Stanley Center for Psychiatric Research, Broad Institute, Cambridge, MA, USA; Program in Medical and Population Genetics, Broad Institute, Cambridge, MA, USA.

Cell reports methods
|December 13, 2023
PubMed
概括

我们使用piggyBac转位子开发了一个CRISPR-Cas9系统,用于快速,大规模的细胞基因组变异工程. 这使得发育和疾病中突变的有效功能研究成为可能.

关键词:
科普:遗传学 在CP:遗传学.CP: 干细胞是一种干细胞.克里斯普尔是什么意思?克里斯普尔是什么意思?一系列的等位基因序列.功能性基因组学 功能性基因组学在gRNA图书馆里.基因组编辑 基因组编辑基因组工程是基因组工程.诱导多能干细胞的诱导干细胞.突变建模的模型.在 piggyBac 转位器上

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