超内皮细胞中性粒细胞迁移通过Piezo1机械感知激活了杀菌功能
Amitabha Mukhopadhyay1, Yoshikazu Tsukasaki1, Wan Ching Chan1
1Department of Pharmacology and Regenerative Medicine and The Center for Lung and Vascular Biology, University of Illinois College of Medicine, Chicago, IL 60612, USA.
Immunity
|December 13, 2023
概括
在多态核白细胞 (PMN) 中,Piezo1感知到的机械力量增强了它们杀死细菌的能力. 这种机械感知对于清除感染和预防严重的肺炎至关重要.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
背景情况:
- 机械力量在通过内皮壁壁迁移期间调节多态核白细胞 (PMN) 功能中的作用尚未完全理解.
- 了解这一过程对于开发抗菌感染策略至关重要.
研究的目的:
- 调查Piezo1机械传感器在跨内皮细胞迁移期间PMN功能中的作用.
- 阐明通过机械力量调节PMN杀菌活性的分子机制.
主要方法:
- 在PMN中Piezo1的遗传删除.
- 在体内感染模型 (Pseudomonas aeruginosa).
- 微流体系统应用定义的机械力.
- 成像和测量杀菌功能的测量.
主要成果:
- 在PMNs中的Piezo1在跨内皮细胞迁移期间感知机械力,诱导信号.
- 缺少Piezo1的PMN显示细菌清除受损,感染易感性增加.
- 激活Piezo1或施加机械力会增强PMN的杀菌活性.
- 皮埃佐1上调NADPH氧化酶4,这是杀死细菌的关键酶.
结论:
- 在内皮细胞转移期间,Piezo1介导的PMN张力的机制感知是关键的宿主防御机制.
- 这一途径增强了PMN的杀菌功能,这对于控制细菌感染至关重要.
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