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在黄尾金鱼 (Seriola lalandi) 中对两种MyoD进行克隆,遗传学和表达分析

Bao Shi¹, Ranran Sun², Xuezhou Liu¹

¹State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China; Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Laoshan Laboratory, Qingdao, Shandong 266237, China.

General and comparative endocrinology

|December 13, 2023

概括

这项研究研究了黄尾金鱼的肌肉生长,确定了MyoD基因表达模式. 较高的MyoD水平与生长激素和IGF-I相关,这对这种有价值的鱼类物种的肌肉发育至关重要.

科学领域:

水产养殖是水产养殖的一种方式.
鱼类生物学鱼类生物学
分子遗传学分子遗传学

背景情况:

黄尾金鱼 (Seriola lalandi) 是一种分布在全球的高价值鱼类.
了解其肌肉发育机制对于水产养殖至关重要.
肌细胞决定蛋白 (MyoD) 基因是脊椎动物肌肉形成的关键调节者.

研究的目的:

为了描述S. lalandi.中的MyoD1和MyoD2基因.
分析MyoD基因在发育过程和不同组织中的表达模式.
研究MyoD表达和生长因子 (如GH和IGF-I) 之间的关系.

主要方法:

对S. lalandi MyoD1和MyoD2开放式读取框架 (ORF) 的序列分析.
在各种组织 (白肌肉,大脑,垂体,肝脏) 和发育阶段对MyoD mRNA表达的定量分析.
MyoD mRNA水平,生长激素 (GH) 和胰岛素类生长因子 (IGF-I) 血水平之间的相关性分析.

主要成果:

在S. lalandi的MyoD1和MyoD2ORF中,编码的蛋白质具有保存的肌源性域.
MyoDs在白肌肉中表达很高,在发育早期显示MyoD1和MyoD2之间的明显表达水平.
在400克,2岁的鱼类中,MyoD表达达到顶峰,并且与GH和IGF-I水平呈正相关.

结论:

MyoD基因在黄尾金鱼的肌肉发育和生长中起着重要的作用.
MyoD异型的差异表达表明肌肉形成中的专门功能.
增长激素和IGF-I与S. lalandi.通过MyoD通路调节肌肉生长有关.

关键词:

克隆和表达方式生长激素生长激素的作用胰岛素类生长因子胰岛素类生长因子髓母细胞确定蛋白质塞里奥拉·拉兰迪 (Seriola lalandi) 是一个有趣的植物.

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