供应对氧化的细菌丰富产生微生物蛋白质的影响:比较固定和氨同化
Haoran Wang1, Lixia Zhang2, Chang Tian2
1CAS Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; College of Life Sciences, Sichuan University, Chengdu 610065, China.
Bioresource technology
|December 13, 2023
概括
源显著影响氧化细菌 (HOB) 中微生物蛋白质的产生. 在这些细菌中,氨同化能比N2固定更有效地提高生物质产量和蛋白质合成.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
背景情况:
- 氧化细菌 (HOB) 对微生物蛋白 (MP) 生产至关重要.
- 了解源效应是优化HOB中MP合成的关键.
- 连续供给料气是HOB的常见种植方法.
研究的目的:
- 调查不同源对HOB的微生物蛋白质生产的影响.
- 为了在HOB中对MP合成进行N2固定与氨同化进行比较.
- 阐明增强MP生产背后的机制.
主要方法:
- 测序批量培养对N2固定和氨同化进行比较.
- 在连续供给料气的情况下培养氧化细菌.
- 对二氧化碳固定,二氧化固定和生物质产量的量化.
主要成果:
- 在基本条件下,N2固定HOB社区在基本条件下显示出显著的CO2和N2固定.
- 实现的最高生物质产量为0.153g CDW/g COD-H2.
- 与N2固定相比,氨同化导致MP合成和生物质积累增加了7.414.3倍.
结论:
- 氨同化是一种比N2固定更有效的策略,用于增强HOB中的MP合成和生物质产量.
- 增强的H2和CO2吸收可能介于氨的积极作用.
- 丰富特定的细菌物种 (例如,Xanthobacter,Acinetobacter) 和增加的酶和谷氨酸合成酶丰富度促进同化并优化MP生产.
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