三螺旋虫 cathepsin B 结合并降解了宿主的肠道I型原蛋白
You Jiao Zao1, Ge Cheng2, Miao Miao Feng2
1Department of Pathogen Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001, PR China; Yunan University School of Medicine, Kunming 650091, PR China.
International journal of biological macromolecules
|December 13, 2023
概括
三螺旋菌 (Trichinella spiralis) 甲素B (TsCB) 降解宿主I型原蛋白,有助于寄生虫侵入肠道细胞. 这一发现为通过针对这种相互作用来治疗寄生虫疾病提供了洞察力.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 食品安全 食品安全
背景情况:
- 三病是一种严重的动物传播疾病,影响人类健康和经济.
- 肠上皮细胞 (IEC) 的幼虫入侵对Trichinella spiralis (T. spiralis) 感染至关重要.
- 螺旋螺旋 (T. spiralis Cathepsin B) (TsCB) 参与促进这种入侵过程.
研究的目的:
- 调查 TsCB 与小鼠 IEC 相互作用并影响的机制.
- 为了识别与 TsCB 相互作用的宿主蛋白.
- 阐明 TsCB 在肠道屏障降解中的作用.
主要方法:
- 对T. spiralis Cathepsin B (TsCB) 的克隆,表达和表征.
- GST拉下测试与LC-MS/MS结合,以识别相互作用的蛋白质.
- 在感染期间对宿主肠道粘膜中TsCB和I型原水平的分析.
主要成果:
- TsCB已成功克隆和表达,表现出囊蛋白酶活性.
- 确定了140种与rTsCB相互作用的蛋白质,包括I型原蛋白.
- TsCB表达增加,而I型原体在感染的肠道粘膜中减少.
- 发现TsCB可以结合并降解宿主I型原蛋白.
结论:
- TsCB通过向I型原蛋白,在降低宿主肠道上皮屏障方面发挥着关键作用.
- 了解这种相互作用为开发抗寄生虫感染的新疗法提供了基础.
- 这项研究有助于澄清T. spiralis的入侵机制.
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