Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

10.0K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Cognitive function depends upon <i>Satb2</i> gene dosage in cortical projection neurons.

bioRxiv : the preprint server for biology·2026
Same author

Type I interferon primes the alveolar epithelium to receive reparative signals from tissue-resident macrophages.

bioRxiv : the preprint server for biology·2026
Same author

IDH1-R132H enhances oncolytic HSV-1 therapy by facilitating viral entry and immune activation in glioma.

Nature communications·2026
Same author

Aging disrupts spatiotemporal coordination in the cycling murine ovary.

Nature aging·2026
Same author

Publisher Correction: Tumor transcriptional state predicts survival in immune-checkpoint-blockade-treated glioblastoma.

Nature cancer·2026
Same author

Tumor transcriptional state predicts survival in immune-checkpoint-blockade-treated glioblastoma.

Nature cancer·2026

相关实验视频

Updated: Jul 8, 2025

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
10:44

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

Published on: March 23, 2022

4.2K

幻灯片标签可用于多模式空间基因组的单核条形码

Andrew J C Russell1,2, Jackson A Weir1,3, Naeem M Nadaf1

  • 1Broad Institute of Harvard and MIT, Cambridge, MA, USA.

Nature
|December 13, 2023
PubMed
概括

滑块标签技术可以精确地绘制完整组织中的单个细胞的空间图. 这项创新与现有的单细胞测试无结合,推进空间基因组学和组织分析.

更多相关视频

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.6K
Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.3K

相关实验视频

Last Updated: Jul 8, 2025

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing
10:44

Low-input Nucleus Isolation and Multiplexing with Barcoded Antibodies of Mouse Sympathetic Ganglia for Single-nucleus RNA Sequencing

Published on: March 23, 2022

4.2K
Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
08:18

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

1.6K
Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.3K

科学领域:

  • 基因组学
  • 分子生物学
  • 生物技术

背景情况:

  • 高通量单细胞技术已经彻底改变了组织生物学,但缺乏常规的空间定位.
  • 精确地绘制组织中的细胞对于理解复杂的生物系统至关重要.

研究的目的:

  • 开发一种多用途的方法,用于在完整的组织中空间定位配置细胞.
  • 整合空间信息与各种单细胞测量.

主要方法:

  • 开发了幻灯片标签:用空间条形码标记完整的组织部分中的单个核.
  • 在小鼠海马体,人类大脑,桃体和黑色素瘤组织上应用Slide-tags.
  • 进行了多原子测量,包括RNA,开放色素和T细胞受体序列.

主要成果:

  • 在小鼠海马体中实现了10微米以下的空间分辨率,具有高质量的全转录组数据.
  • 在人体组织中揭示了细胞类型特定的基因表达模式和空间上背景化的受体-连接体相互作用.
  • 在不同的黑色素瘤微环境中驱动癌细胞转变的转录因子.

结论:

  • 幻灯片标签为空间基因组学提供了一个通用平台,可适应多种单细胞测试.
  • 可以将已建立的单细胞测量集成到空间领域.
  • 促进组织结构和细胞相互作用的研究.