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含有SLCG_7083调节器的PAS域参与Streptomyces lincolnensis的形态发育和葡萄糖利用

Chun-Yan Lin¹, Yixian Ru¹, Yanchao Jin²

¹College of Materials and Chemical Engineering, Minjiang University, Fuzhou, 350108, China.

Microbial cell factories

|December 14, 2023

概括

一种新发现的Streptomyces lincolnensis蛋白,SLCG_7083,调节葡萄糖的使用和发育. 这种Per-Arnt-Sim (PAS) 域蛋白质影响生长,胞和新陈代谢,为Streptomyces调节提供了新的见解.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

链杆菌 (Streptomyces lincolnensis) 是抗生素lincomycin的一个关键生产者.
虽然林氏素生物合成得到了充分的研究,但S. lincolnensis中初级代谢调节仍然不太了解.

研究的目的:

研究SLCG_7083基因及其蛋白质产物在Streptomyces lincolnensis中的功能.
阐明SLCG_7083在初级代谢和形态发育中的作用.

主要方法:

在SLCG_7083.3.的基因失活.
进行比较的转录基因分析.
对Per-Arnt-Sim (PAS) 域蛋白质功能的分析.

主要成果:

SLCG_7083的不活化促进了葡萄糖的利用,减缓了菌根的生长,并影响了子.
转录基因分析显示,SLCG_7083抑制体积,细胞分裂和脂质代谢基因.
SLCG_7083激活了参与碳代谢的基因.

结论:

SLCG_7083是一种含有PAS域的调节器,控制S. lincolnensis的形态发育和葡萄糖利用.
这项研究揭示了SLCG_7083.3的调节功能.
这些发现为Streptomyces中SLCG_7083类蛋白质的转录效应提供了新的见解.

关键词:

葡萄糖利用率的使用情况形态发展的形态发展.PAS 域名 PAS 域名这种植物是Streptomyces lincolnensis.转录调节器的调节器

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