发现和结构阐明从虫肠道Kitasatospora胞中的糖和5-基米格拉斯塔丁
Ji Hyeon Im1, Seoyoung Oh1, Eun Seo Bae1
1N atural Products Research Institute, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.
Journal of industrial microbiology & biotechnology
|December 14, 2023
概括
研究人员发现了两种新型的密格拉斯塔丁衍生物,即glycosyl-migrastatin和5-hydroxy-migrastatin,来自于便甲虫中发现的肠道细菌. 这些发现扩大了已知的migrastatin家族,并提供了对微生物天然产品生物合成的见解.
科学领域:
- 自然产品化学 自然产品化学
- 微生物生物化学 微生物生物化学
- 有机化学 有机化学
背景情况:
- 密格拉斯塔丁家族包括具有潜在生物活动的宏环乳.
- 昆虫的肠道微生物群是新型二次代谢物的丰富来源.
- 基塔萨托类物种是已知的生物活性化合物的生产者.
研究的目的:
- 从Kitasatospora sp.中分离和描述新的二次代谢物. JL24.JL24.JL24.JL24.JL24.JL24.JL24.JL24.JL24.JL24.JL24.JL.
- 阐明新型米格拉斯塔衍生物的结构和绝对配置.
- 为了研究产生细菌的生物合成潜力.
主要方法:
- 使用色谱技术将代谢物分离和净化.
- 通过全面的光谱分析 (NMR,MS,UV) 阐明结构.
- 使用莫舍尔法和化学衍生物的绝对配置的确定.
主要成果:
- 鉴定出了两种新的密格拉斯塔丁衍生物,即甘氨基-密格拉斯塔丁 (1) 和5-基-密格拉斯塔丁 (2).
- 化合物1代表了第一个已知的糖化密格拉斯塔丁,具有d-葡萄糖部分.
- 化合物2具有一个新的氧基团和一个未报告的性中心.
- 基因组分析表明一种I型多基酸合成酶途径.
结论:
- 葡萄糖-米格拉斯塔丁和5-基-米格拉斯塔丁的发现扩大了米格拉斯塔丁的结构多样性.
- 这项研究强调了昆虫肠道细菌作为新型天然产品来源的潜力.
- 鉴定出的生物合成基因集群为了解米格拉斯塔丁生产提供了基础.
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