开发一种低污染介质,用于培养乳酸细菌
Xóchitl Nochebuena-Pelcastre1, Ana Karen Álvarez-Contreras2, Marcos Francisco Hernández-Robles2
1Departamento de Biotecnología, División de Ciencias Biológicas y de La Salud, UAM Iztapalapa, Av. Sn. Rafael Atlixco 186, Vicentina, CP 09340, Iztapalapa, Mexico.
Heliyon
|December 14, 2023
概括
为乳酸细菌 (LAB) 生产开发具有成本效益的培养基至关重要. 这项研究评估了新媒体配方,发现MX和MXC支持以降低成本高效的LAB生产.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 发酵技术的发酵技术
背景情况:
- 富含蛋白质的介质对于乳酸细菌 (LAB) 生产至关重要,但会增加成本.
- 优化媒体组合是降低生产成本,同时保持效率的关键.
研究的目的:
- 制定和评估用于乳酸细菌 (LAB) 生产的新型培养基.
- 根据消费效率 (E),产量生产 (Y) 和特定基质消费率 (qS) 来评估媒体性能.
主要方法:
- 评估了四种培养介质:Man Rogosa Sharp (MRS),卷心菜液 (MC),新平衡介质 (MX) 和MXC (含卷心菜液的MX).
- 测试的介质是使用Lactobacillus acidophilus ATCC 4356和Lactiplantibacillus plantarum ATCC 10241. 这两种基因.
- 分析了葡萄糖消耗效率 (EGLU),生物质产量 (YX/S) 和乳酸产量 (YLAC/S).
主要成果:
- 在MRS,MX和MXC中,Lactiplantibacillus plantarum显示出100%的葡萄糖消耗效率.
- 在MRS和MX (0.02 ± 0.003) 中,生物质产量 (YX/S) 是可比的.
- 在MRS (0.57 ± 0.03) 和MX (0.51 ± 0.02) 中,乳酸产量 (YLAC/S) 高. 此外,MXC还显示出显著的乳酸产量 (0.47 ± 0.04).
结论:
- 新的平衡培养基 (MX) 和MXC是有效生产乳酸细菌 (LAB) 的可行,低成本替代品.
- 这些发现支持LAB.的经济发酵过程的发展.
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