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Updated: Jul 8, 2025

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通过基于spike-in的规范化和预测时间年龄来分析NAD封顶RNA的Epitranscriptome分析

Dean Li^1,2, Shuwen Ge^1,2, Yandong Liu³

¹Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 100 Hai Ke Road, Pudong, Shanghai 201210, China.

|December 14, 2023

概括

这项研究介绍了enONE,一个用于分析尼古丁胺氨酸二核酸-RNA (NAD-RNA) 修改的计算工具. enONE减少了技术噪音,使得与年龄相关的NAD-RNA变化的发现和基于RNA的新型衰老时钟成为可能.

科学领域:

史诗转录组学史诗转录组学
计算生物学计算生物学
分子生物学分子生物学

背景情况:

尼古丁胺胺氨基二核酸 (NAD) 启动RNA转录,形成NAD覆盖RNA (NAD-RNA).
NAD-RNA是一种未经研究的表体转录基因修饰,将代谢物与转录物联系起来.
现有的NAD-RNA分析方法复杂,容易产生批量效应,并阻碍定量分析.

研究的目的:

开发一个计算框架,enONE,用于强大的NAD-RNA分析.
为了减轻NAD-RNA分析中的技术噪音和批量效应.
为了能够在数据集中对NAD-RNA进行定量和比较评估.

主要方法:

开发enONE计算框架的开发.
使用设计的spike-inRNA进行正常化.
实施模块化规范化程序和评估指标.
适用于人类衰老队列.

主要成果:

在NAD-RNA数据中,enONE有效地减轻了技术噪音和批量效应.
在人类老龄化队列中发现了与年龄相关的NAD限制特征.
开发了一种基于RNA的新型衰老时钟,集成了转录组和NAD修饰的表转录组数据.

结论:

enONE为准确的定量NAD-RNA分析提供了基础.
这项研究揭示了在衰老过程中对NAD-RNA动态的见解.
enONE促进了对生理变化有反应的NAD-RNA的发现,支持功能性研究.

关键词:

计算生物信息学是指计算机生物信息学.生物科学中的方法论.序列分析是指进行序列分析.文字转录学 (Transcriptomics) 是一个学科.

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