TFE3在调解骨肌肉线粒体适应以进行运动训练中的作用
Jenna C Wong1, Ashley N Oliveira1, Priyanka Khemraj1
1Muscle Health Research Centre, School of Kinesiology and Health Science, York University, Toronto, Ontario, Canada.
Journal of applied physiology (Bethesda, Md. : 1985)
|December 14, 2023
概括
转录因子E3 (TFE3) 对于骨肌肉的运动适应至关重要,增强线粒体功能和耐力. 它的缺失阻止了这些有益的训练效应,突出了TFE3的作用.
科学领域:
- 运动生理学 运动生理学
- 线粒体生物学 线粒体生物学
- 骨肌肉的新陈代谢
背景情况:
- 转录因子E3 (TFE3) 调节了 lysosomal 基因,并参与了 mitofgy.
- 在骨肌肉适应运动时,TFE3的确切作用尚不清楚.
- TFE3有可能参与优化身体活动期间的线粒体质量控制.
研究的目的:
- 研究TFE3在骨肌肉适应耐力运动中的作用.
- 为了确定TFE3缺乏对肌肉疲劳,收缩性质和训练后线粒体功能的影响.
- 阐明将TFE3与运动诱导的线粒体改善联系起来的分子机制.
主要方法:
- 在野生类型和TFE3淘汰赛小鼠中进行耐力训练 (自愿轮子运行).
- 在位置刺激坐骨神经以评估肌肉疲劳和收缩功能.
- 测量线粒体呼吸,细胞染色体c氧化酶活性和蛋白质表达.
- 通过使用胆固醇治疗评估自和髓流动.
主要成果:
- 运动训练改善了野生类型小鼠的肌肉疲劳性和线粒体氧气消耗.
- 训练有素的野生型小鼠显示线粒体含量增加 (COX活性和COX I表达).
- 这些训练适应在TFE3淘汰赛小鼠中被取消,尽管正常的线粒诱导.
结论:
- TFE3对于有益的骨肌肉适应耐力运动至关重要,包括增强线粒体功能和含量.
- 缺少TFE3会损害运动训练的益处,这表明TFE3的作用超出了线粒的范围.
- TFE3代表了改善骨肌功能和代谢健康的潜在治疗标.
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