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在Staphylococcus aureus生物膜感染期间,阐明颗粒细胞髓衍生的抑制细胞异质性

Blake P Bertrand¹, Cortney E Heim¹, Scott A Koepsell¹

¹Department of Pathology, Microbiology, and Immunology, University of Nebraska Medical Center, 985900 Nebraska Medical Center, Omaha, NE 68198-5900, United States.

Journal of leukocyte biology

|December 14, 2023

概括

在假肢关节感染 (PJI) 期间,发现了一种新型的骨髓衍生抑制细胞 (MDSC) 现型,F4/80+MHCII+MDSCs. 这些细胞表现出免疫抑制性质,并表明PJI中的颗粒细胞转变为单细胞细胞.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
传染性疾病传染性疾病

背景情况:

骨髓衍生抑制细胞 (MDSC) 是免疫抑制细胞,在癌症和假肢关节感染 (PJI) 等慢性炎症期间扩大.
MDSCs被广泛分为单细胞 (M-MDSCs) 和粒细胞 (G-MDSCs) 种群.
G-MDSCs是PJI中占主导地位的白细胞类型,但它们的体内维持和异质性仍然不清楚.

研究的目的:

在假肢关节感染 (PJI) 中识别和描述新的MDSC种群.
在PJI期间调查MDSC的细胞异质性和潜在可塑性.

主要方法:

使用表面标记表达 (Ly6G,Ly6C,F4/80,MHCII) 来识别MDSC种群.
通过测序 (CITE-seq) 来对转录组和表位组进行细胞索引的转录组分析.
在PJI的小鼠模型中进行采养转移实验,以评估MDSC可塑性.

主要成果:

在PJI中确定了一种以前未被识别的MDSC群体 (Ly6G+Ly6C+F4/80+MHCII+),显示出ex vivo免疫抑制活性.
这些F4/80+MHCII+MDSC具有独特的转录基因特征,与细胞因子反应,炎症细胞死亡和单核细胞分化相关的途径增加.
通过收养转移的G-MDSCs表现出可塑性,其中一个子集在PJI期间体内获得F4/80和MHCII表达.

结论:

这项研究揭示了PJI中的新型F4/80+MHCII+MDSC表型,有助于理解MDSC异质性.
这些发现表明,在PJI等生物膜感染期间,MDSC中存在潜在的颗粒细胞到单细胞的转变.
这一发现为研究PJI中MDSC功能和治疗向开辟了新的途径.

关键词:

黄金色的S. 黄金色的在CITE-seqq.在G-MDSCC中使用.文字转录学 (Transcriptomics) 是一个学科.

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