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研制的伪结可以刺激-1编程的核糖体框架转移或阻止RNA病毒中的编码子读取

Xiaolan Huang¹, Zhihua Du²

¹School of Computing, Southern Illinois University at Carbondale, Carbondale, IL, USA.

Journal of biomolecular structure & dynamics

|December 14, 2023

概括

研究人员发现了新的,复杂的RNA伪节点,调节病毒编程的核糖体框架转移并阻止密码子的读透,扩大了这些功能性RNA结构的已知的曲目.

科学领域:

分子生物学分子生物学
病毒学病毒学
RNA结构和功能 RNA结构和功能

背景情况:

RNA伪结对于病毒功能至关重要,例如编程的核糖体框架转移 (-1 PRF) 和停止密码子读透 (SCR).
已知有三根的伪结 - - 在如SARS-CoV-2等冠状病毒中是1PRF刺激器.
伪结结构在尺寸和复杂性方面表现出显著的多样性.

研究的目的:

调查先前识别的-1 PRF或SCR刺激伪结中精细结构的潜力.
为了识别除了基本的茎环形成之外,具有额外结构元素的伪结.
探索这些复杂的RNA架构的结构可行性和影响.

主要方法:

重新检查现有的1PRF和SCR刺激型伪节点,重点关注连接环较长的假节点.
识别额外的结构元素,如茎环,嵌入的伪结,亲吻针头和环-环相互作用.
计算模型构建以评估结构可行性并发现隐藏的特征.

主要成果:

发现了许多精心制作的伪结,在各种病毒家族中包含了新的结构动机.
识别独特的结构,包括嵌入式双伪结和由CPK-1介导的紧循环-循环伪结.
建模证实了结构完整性,并揭示了这些复杂的伪结的潜在功能方面.

结论:

这项研究显著扩大了已知的功能性RNA伪结的多样性,这些假结参与了病毒重编码机制.
这些发现有助于我们更好地了解RNA结构生物学以及1PRF和SCR的调节.
发现的精心设计的伪结为基于RNA的疗法和人工RNA分子提供了新的目标.

关键词:

结构 RNA 结构 RNA 结构 RNA病毒的RNA病毒亲吻的环节吻的环节伪结伪结伪结重编码是指重新编码.停止编码器读取通过.−1 编程的核糖体框架转移.

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