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蛋白质纳米颗粒上的抗原间距会影响抗体对疫苗接种的反应

Daniel Ellis¹, Annie Dosey², Seyhan Boyoglu-Barnum³

¹Institute for Protein Design, University of Washington, Seattle, WA 98195, USA; Department of Biochemistry, University of Washington, Seattle, WA 98195, USA; Graduate Program in Molecular and Cellular Biology, University of Washington, Seattle, WA 98195, USA.

|December 14, 2023

概括

设计具有精确间距流感血素受体结合域 (RBDs) 的纳米粒子疫苗可以增强抗体反应. 这种方法提高了针对不同流感菌株的疫苗疗效,为下一代病毒疫苗铺平了道路.

科学领域:

疫苗学疫苗学疫苗学
结构生物学结构生物学
计算式蛋白质设计

背景情况:

下一代疫苗需要免疫原设计,精确控制抗体反应.
流感血凝素 (HA) 的受体结合域 (RBD) 是流感疫苗的关键标.
控制免疫原上的抗原呈现对于产生有效的免疫力至关重要.

研究的目的:

开发一个纳米粒子疫苗平台,以精确控制抗原构成和间距.
研究抗原间隔对抗体对抗流感HA的反应的影响.
建立抗原间距作为基于结构的疫苗设计中的关键参数.

主要方法:

利用计算式蛋白质设计创建了一个展示HA RBDs的纳米粒子平台.
在单体或三体形式的工程HA RBD,与可调节间距的纳米颗粒相连.
评估了抗体反应,包括血液凝结抑制,中和功效和结合宽度.

主要成果:

纳米颗粒免疫原体与三重体HA RBDs之间的间距减少引起了优异的抗体反应.
经过优化间距观察到改善的凝血抑制和中和功效.
在各种H1 HA亚型中实现了增强的结合宽度.

结论:

开发的开发的开发的
三头三头的人.
纳米粒子平台可以精确控制疫苗设计的抗原间距.
抗原间距是影响抗HA抗体反应质量和范围的重要因素.
该平台为开发下一代流感疫苗和潜在的其他病毒疫苗提供了一个有前途的战略.

关键词:

CP: 免疫学免疫学抗原的设计设计.冷电子显微镜的使用方法血凝氨酸是什么血凝氨酸流感流感流感流感流感纳米颗粒是一种纳米粒子.蛋白质设计蛋白质设计疫苗疫苗疫苗疫苗

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