相关实验视频

Updated: Jul 8, 2025

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

用CRISPR-CasPR精确编辑内源性Chlamydomonas reinhardtii基因的协议

Adrian Pascal Nievergelt¹, Dennis Ray Diener², Aliona Bogdanova²

¹Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstraße 108, 01307 Dresden, Germany; Human Technopole, V.le Rita Levi-Montalcini, 1, 20017 Milan, Italy.

|December 14, 2023

概括

这项研究引入了一种有效的CRISPR-Cas基因编辑协议,用于Chlamydomonas reinhardtii,使无痕的诺金突变和蛋白质修饰成为可能. 这种新方法提高了克拉米多莫纳斯基因组工程的整合效率.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
藻类研究研究藻类研究

背景情况:

克里斯普尔-Cas基因组工程在克拉米多莫纳斯强化传统上面临着低的整合效率.
已建立的基因工具存在于Chlamydomonas,但高效的基因编辑一直是一个瓶.

研究的目的:

提出一个改进的协议,以高效的同质导向的诺基因突变发生在Chlamydomonas reinhardtii.
为了使大多数蛋白质的融合标签和序列修改无痕整合.
通过高效的交叉和强大的查,促进基因编辑.

主要方法:

开发一种基于CRISPR-Cas的新型协议,用于克拉米多莫纳斯病毒.
使用同质导向修复来实现精确的基因整合.
实施有效的交叉和选策略进行基因分析.

主要成果:

在常见的 Chlamydomonas 菌株中实现了高整合效率的 knockin 突变发生.
证明了融合标签和序列修改的无痕集成.
成功地应用了协议,而不需要先前存在的突变系.

结论:

提出的协议显著提高了克里斯普尔-卡斯基因组工程在克拉米多马斯的效率.
在这种藻类模型中,这种方法为无痕的基因改造和蛋白质工程提供了一种多功能工具.
该协议赋予研究人员强大的基因操纵能力,用于克拉米多蒙纳斯.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?细胞生物学细胞生物学遗传学是一个遗传学.分子生物学分子生物学

更多相关视频

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...