抗生素的细胞质积累和透性在克拉姆阳性和克拉姆阴性细菌中,通过化标记策略实时可视化
Scott O Squire1,2, Sepehr Sebghati1,2, Ming C Hammond1,2
1Department of Chemistry, University of Utah, Salt Lake City, Utah 84112, United States.
ACS chemical biology
|December 14, 2023
概括
这项研究引入了一种新的活细胞测定方法,用于跟踪化合物进入细菌的过程. 该方法允许实时测量格拉姆阳性和格拉姆阴性细菌中的抗生素积累.
科学领域:
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
- 生物技术是生物技术.
背景情况:
- 了解化合物如何进入细菌细胞对于开发新的抗生素至关重要.
- 现有的测量化合物积累的方法通常涉及细胞处理,这可能会改变结果.
- 需要实时活细胞检测来准确评估细菌化合物的透性.
研究的目的:
- 开发和验证一种新的实时活细胞测定方法,用于测量细菌中的化合物积累和透性.
- 为了可视化活细菌细胞细胞质中的复合动力学.
- 为了比较克拉姆阳性和克拉姆阴性细菌中抗生素的细胞内积累.
主要方法:
- 利用一种新的化标记策略,直接可视化化合物积累.
- 运用流动细胞计量用于细胞内化合物度的定量测量.
- 在模型细菌中建立了测试:大肠杆菌 (E. coli) 和细菌细菌 (B. subtilis).
主要成果:
- 该试验能够直接实时可视化活细菌细胞质中的化合物积累动态.
- 量化测量可以通过流细胞计进行复制,即使在低度的化合物中也是如此.
- 通过比较细胞内积西普洛素和安皮西林来确定试验的实用性.
结论:
- 这种新型化剂试验为实时研究细菌化合物的透性提供了强大的工具.
- 这种分析比传统的基于质谱法 (MS) 的方法对低度化合物具有更高的灵敏度.
- 它有助于直接比较不同细菌物种的抗生素积累.
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