干细胞表达的类似DEVIL的小在非生物压力下通过abscisic acid信号传递维持根生长
Liyun Yang1, Xiaochen Hu1, Mengfei Ren1
1College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China.
Plant physiology
|December 14, 2023
概括
五个DEVIL-like (DVL) 基因调节植物根干细胞的维护. 这些的功能是冗余的,影响酸 (ABA) 信号,以控制压力下的根生长.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 发展生物学 发展生物学
背景情况:
- 干细胞对于植物生长和发育至关重要.
- 在阿拉比多普西斯根干细胞维护中的DEVIL-like (DVL) 小基因的功能是未知的.
- DVL基因优先表达在阿拉比多普西斯根的静止中心.
研究的目的:
- 为了研究5个DEVIL-like (DVL) 小基因在Arabidopsis thaliana根部发育中的功能.
- 阐明DVL在干细胞维护中的作用及其与酸 (ABA) 信号的相互作用.
- 了解DVL基因如何促进植物对非生物压力的反应.
主要方法:
- 数据挖掘用于识别DVL基因.
- 基因过度表达和突变生成 (单个和五重突变).
- 在正常条件下和含有ABA的条件下进行转录组分析,RT-qPCR和表型分析.
主要成果:
- 过度表达DVL基因导致根长度缩短和发芽表型.
- 五倍的DVL突变显示出比野生类型 (WT) 略长的根.
- DVL调节ABA信号基因;DVL20过度表达的植物显示出ABA耐受性增加,而五重突变体显示出敏感性增加.
结论:
- DVL1,2,5,8和20在维护根干细胞方面有多余的功能,特别是在非生物压力下.
- 这一功能是通过调节酸 (ABA) 信号通路来调节的.
- 调节ABA信号组件可以拯救DVL相关的根生长缺陷,并赋予ABA耐受性.
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