详细比较内部开发和商用心脏切割和选择性全面的二维液态色谱系统
Marie Pardon1, Rafael Reis2, Peter de Witte3
1Laboratory for Pharmaceutical Analysis, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Herestraat 49 Box 824, 3000 Leuven, Belgium; Laboratory for Molecular Biodiscovery, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven, Herestraat 49 Box 824, 3000 Leuven, Belgium.
Journal of chromatography. A
|December 14, 2023
概括
本研究比较了内部开发和商用二维液态染色学 (2D-LC) 系统,用于复杂的样本分析. 商业系统提供了用户友好性和高级功能,如活性溶剂调制 (ASM),而内部设置为特定应用提供了成本效益.
科学领域:
- 分析化学 分析化学
- 染色体学 染色体学是什么
- 分离科学 分离科学
背景情况:
- 二维液态色谱 (2D-LC) 越来越受欢迎,用于分析复杂的样品.
- 商用2D-LC系统提供了用户友好的操作和专用软件,与旧的内部设置形成鲜明对比.
- 水友交互液体染色学 (HILIC) 和逆相LC (RPLC) 之间的移动相不兼容是一个关键的挑战.
研究的目的:
- 将内部开发的2D-LC设置与商用仪器进行比较.
- 为了评估实验差异,成本,灵活性和操作方便.
- 评估在HILIC-RPLC分离中管理移动相不兼容性的策略.
主要方法:
- 内部2D-LC设置 (限制毛细血管和陷柱) 与使用活性溶剂调制 (ASM) 的商业系统的比较.
- 对覆盖广泛极性范围的28种化合物样本混合物的分析.
- 在二维 (2D) 染色体中评估峰值形状和恢复.
主要成果:
- 商用2D-LC系统与选择性综合2D-LC实现了优越的峰值形状和早期化化合物的回收,尽管分析时间较长.
- 商业系统中的活性溶剂调制 (ASM) 有效地解决了移动相不兼容问题.
- 使用限制毛细血管和陷柱的内部设置是特定目标分析的可行,具有成本效益的替代方案.
结论:
- 内部的2D-LC系统可用于分析特定的目标化合物或样品.
- 商用2D-LC仪器更适合用于更复杂的分析挑战,需要先进的功能.
- 在内部和商业2D-LC之间的选择取决于具体的应用需求和分析目标.
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