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用光子计数X射线计算机断层扫描直接比较两种水溶液的异酸和异酸玻璃化

Jason T Parker¹, Anthony N Consiglio¹, Boris Rubinsky¹

¹Department of Mechanical Engineering, University of California, Berkeley, Berkeley, CA, 94720, USA.

|December 14, 2023

概括

光子计数X射线CT揭示了冰在封闭系统中的玻璃化过程中明显的冰形态. 当边界效应受到控制时,在同位体和同位体条件下形成类似的冰块.

科学领域:

低温生物学低温生物学
生物物理学的生物物理.
材料科学材料科学材料科学

背景情况:

玻璃化可以实现无冰的冷保存,但受到研究内部过程的工具的限制.
隔离室通常是不透明的,阻碍了对玻璃化的直接观察.
现有的对同和同玻璃化进行比较缺乏受控的边界条件.

研究的目的:

介绍光子计数X射线计算机断层扫描 (CT) 用于研究玻璃化.
在等离子和等离子条件下比较冰的形成和玻璃化.
研究封闭式与非封闭式冷却对水溶液的影响.

主要方法:

利用光子计数X射线CT用于高分辨率成像.
采用可移动的活塞来模拟等离体系统中等离体边界条件.
在受控条件下冷却50%重量DMSO和珊瑚玻璃化溶液 (CVS1).

主要成果:

类似的冰和蒸汽体积分数在等离体和等离体冷却过程中形成.
在等离体系统中观察到明显的冰和蒸气腔形态.
由于快速冷却和压力下降,在封闭系统中可能出现蒸汽脱气或空洞化.

结论:

当控制边界效应时,异和异条件产生可比的冰分数.
封闭式玻璃化表现出独特的冰/蒸气形态,表明复杂的热流体动力学.
光子计数X射线CT对于在封闭系统中的基本玻璃化研究是有价值的.

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