猪多能干细胞快速转化为巨细胞,在化学定义条件下
Xiaolong Wu1, Yu Ni2, Wenhao Li1
1College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering and Technology, Northwest A&F University, Yangling, Shaanxi, China.
The Journal of biological chemistry
|December 14, 2023
概括
研究人员开发了一种快速的11天方法,从干细胞中制造猪巨细胞. 这些干细胞衍生的巨体模仿初级细胞,为研究宿主-病原体相互作用提供了可再生资源.
科学领域:
- 干细胞生物学 干细胞生物学
- 免疫学 免疫学 免疫学
- 兽医医学 兽医医学 兽医医学
背景情况:
- 主要猪膜巨细胞 (PAMs) 对于研究宿主-病原体相互作用至关重要,但很难获得.
- 为了进行一致的研究,需要一种可再生的猪巨体体外来源.
研究的目的:
- 开发一种有效的协议,从猪多能干细胞 (pPSCs) 获得巨细胞.
- 描述这些pPSC衍生巨细胞 (pPSCdMs) 的分子和功能性质.
- 研究参与pPSC分化成巨细胞的信号通路.
主要方法:
- 开发了一个为期11天的协议,将pPSC分化为巨细胞.
- 分析了pPSCdM表面蛋白和转录因子表达.
- 评估了功能特征,包括内细胞,细胞和对LPS的反应.
- 进行了转录组测序,以在分化过程中识别关键信号通路.
- 研究了转化生长因子β (TGF-β) 信号传递的作用.
主要成果:
- pPSCdM的分子形状与PAMs相似,包括特定的表面蛋白和转录因子.
- pPSCdMs表现出关键的巨细胞功能:内细胞,细胞和对LPS刺激的反应.
- pPSCdM对猪呼吸和生殖综合征病毒 (PRRSV) 感染敏感.
- 转录组分析显示,TGF-β信号传递对中皮形成至关重要.
- 在造血内皮阶段抑制TGF-β信号增强了造血.
结论:
- 建立了一个高效,快速的协议,在11天内从pPSC中产生功能成熟的猪巨细胞.
- 在宿主-病原体相互作用研究中,pPSCdM代表了对初级PAM的有希望的,可再生的替代品.
- 了解TGF-β信号提供了优化巨细胞与PSC差异化的见解.
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